【摘要】 目的 对深圳市某花鸟市场鹦鹉热衣原体(Chlamydia psittaci，Cps)带菌情况进行筛查，并针对筛查阳性的样本进行全面解析，为鹦鹉热的流行病学研究、疾病防控及公共卫生管理提供科学依据。方法 2023年5月和2024年6月于深圳市某花鸟市场鹦鹉专卖档口开展2次采样调查工作，对采集的样本进行Cps筛查，阳性样本通过细胞培养的方式分离Cps，对分离出的Cps进行全基因组测序，并进行基因组学分析，探究基因型分布及进化关系。结果 共采集110份样本，其中38份样本呈Cps阳性，阳性率为34.5%(38/110)。成功从1只Cps阳性鹦鹉的小肠黏膜中分离出1株Cps。通过全基因组测序获得该分离株完整基因组序列，并鉴定为A基因型。系统发育进化树分析显示，该株Cps与鹦鹉来源的参考株在遗传上关系最为接近，提示其可能具有一定的宿主适应性。结论 深圳市某花鸟市场鹦鹉专卖档口采集的样本中Cps检出率较高，应加强花鸟市场，尤其是鹦鹉档口的卫生管理，强化对Cps的持续监测。更多还原

【Abstract】 Objective To screen for the presence of Chlamydia psittaci (Cps) in parrots from bird and flower markets in Shenzhen,and to conduct a comprehensive analysis of the positive samples,providing a scientific basis for epidemiological research,disease prevention and control,and public health management.Method In May 2023 and June 2024,two rounds of investigation and sampling were conducted at a parrot retail stall in a wholesale agricultural market in Shenzhen.The collected samples were screened for Cps,and Cps-positive samples were subjected to isolation through cell culture.Whole-genome sequencing was performed on the isolated Cps strains,followed by genomic analysis to explore genotype distribution and evolutionary relationships.Result A total of 110 samples were collected,of which 38 tested positive for Cps,with a positivity rate of 34.5% (38/110).One Cps strain was successfully isolated from the intestinal mucosa of a Cps-positive parrot.Whole-genome sequencing yielded the complete genome of the isolate,which was identified as genotype A.Phylogenetic analysis revealed that this strain is genetically closest to reference strains derived from parrots,suggesting potential host adaptation.Conclusion A relatively high detection rate of Cps was observed in samples collected from the parrot retail stall in a wholesale agricultural market in Shenzhen.It is recommended to strengthen hygiene management in bird and flower markets,especially at parrot stalls,and to enhance ongoing surveillance of Cps.更多还原