【摘要】 目的 研究黑醋栗对高脂膳食小鼠脂质代谢和肠道菌群的影响。方法 选用C57BL/6J小鼠，雌雄各半，7～8周龄，150只。随机选出12只，普通饲料喂养作为空白对照组(ND组),其余小鼠高脂饲料(60%脂肪供能)喂养8周，筛选肥胖造模成功小鼠按性别分别随机分为4组，高脂对照组(HFD组，生理盐水)、低(LOW组，1 250 mg/(kg·d))、中(MID组，2 500 mg/(kg·d))、高剂量组(HIGH组，5 000 mg/(kg·d))每组6只，共60只。黑醋栗冻干粉连续灌胃10周。结合体质量、血脂、脂肪质量和肠道菌群评价干预效果。结果 采用■描述，用方差分析进行组间差异性检验(α=0.05)。结果ND雌组肝脏质量、肝脏系数、肾脏质量与HFD雌组及各受试物组有差异(P<0.05);ND雄组皮下、肾周、睾周脂肪质量、肠系膜脂肪质量与HFD雄组及LOW雄组有差异(P<0.05);ND雌组小鼠皮下、肾周脂和子宫脂肪质量均与HFD雌组及LOW雌组有差异(P<0.05);ND雄组TC、TG、HDL-C和LDL-C与HFD雄组和HIGH雄组有差异(P<0.05),ND雌组TG、LDL-C与HFD雌组、LOW雌组和HIGH雌组有差异(P<0.05);各组雄鼠ALT有差异(P<0.05);ND组肝组织和脂肪组织细胞完整，HFD组细胞脂肪空泡大，各受试物组细胞有恢复正常水平趋势；各组小鼠β多样性差有差异(P<0.01);厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidota)、疣微菌门(Verrucomicrobiota)等丰度改变(P<0.05)。结论 黑醋栗能降低体脂与内脏脂肪；调节血脂代谢；保护肝功能；调节肠道菌群。更多还原

【Abstract】 Objective To investigate the effects of blackcurrant on lipid metabolism and intestinal flora in High-Fat Diet mice. Methods A total of 150 C57BL/6J mice aged 7-8 weeks were selected, with half male and half female. Twelve mice were randomly selected and fed a normal diet as a blank control group. The other mice were fed a high-fat diet for 8 weeks, and the mice with successful obesity modeling were selected and randomly grouped according to gender. The 60 mice were divided into four groups, including high-fat(normal saline), low-dose(1 250 mg/(kg·d)), medium-dose(2 500 mg/(kg·d)), and high-dose(5 000 mg/(kg·d)), with six mice in each group. The subjects were given continuous gavage for 10 weeks. To evaluate the effect of the intervention and explore the possible mechanism of the intervention. The results were described by ■. Differences between groups were tested by ANOVA(α=0.05). Results There were significant differences in liver weight, liver coefficient and kidney weight between ND female group and HFD female group and each subject group(P<0.05). The subcutaneous fat mass, perirenal fat mass, perirenal fat mass, and mesenteric fat mass in the ND male group were significantly different from those in the HFD male group and the LOW male group(P<0.05). The subcutaneous fat mass, perirenal fat mass and uterine fat mass of ND female mice were significantly different from those of HFD female group and LOW female group(P<0.05). There were significant differences in TC, TG, HDL-C and LDL-C between the ND male group and the HFD male group and the HIGH male group(P<0.05), and there were significant differences in TG and LDL-C between the ND female group and the HFD female group, the LOW female group and the HIGH female group(P<0.05). There was a significant difference in ALT among the groups(P<0.05). The cells of the liver tissue and adipose tissue in the ND group were intact, and the cells in the HFD group had large fat vacuoles, and the cells in each test group tended to return to normal levels. There was a significant difference in β-diversity among the four groups(P<0.01). The changes in the abundance of Firmicutes, Bacteroidota, and Verrucomicrobiota were also observed(P<0.05). Conclusion Blackcurrant can reduce body fat and visceral fat, regulating blood lipid metabolism, protect liver function and regulate intestinal flora.更多还原