【摘要】 目的 研究超活化血小板裂解液（super-activated platelet lysate, sPL）与Bio-Oss骨粉联合应用对大鼠牙周加速成骨正畸（periodontally accelerated osteogenic orthodontics, PAOO）术后牙槽骨改建的影响。方法 选择24只8周龄雄性SD大鼠于右上第一磨牙腭侧建立PAOO牙齿移动模型，随机分为3组：骨皮质切开术组（A组）；骨皮质切开术+Bio-Oss植入组（B组）；骨皮质切开术+Bio-Oss+sPL注射组（C组）。在正畸加力后的第7、14、21、28、56天分别测量各组牙齿移动距离，计算移动速率；于第4周、8周分别从B组、C组中随机抽取4只大鼠处死并采集上颌骨标本，Micro-CT评估右上第一磨牙PAOO术区成骨情况，HE染色观察组织形态变化。结果 三组大鼠牙齿移动距离及速率差异无统计学意义（P>0.05）。术后4周C组BV/TV测量值高于B组（P<0.05）,Tb.Sp低于B组（P<0.05）,BMD、Tb.N、Tb.Th两组差异无统计学意义（P>0.05）。术后8周，C组BV/TV、BMD高于B组，Tb.Sp低于B组，差异有统计学意义（P<0.05），两组Tb.N、Tb.Th差异无统计学意义（P>0.05）。组织学染色显示C组新生骨组织较B组多，骨改建更活跃。结论 sPL与Bio-Oss骨粉联合应用能促进大鼠PAOO术后成骨。更多还原

【Abstract】 Objective To study the effect of the combined application of super-activated platelet lysate(sPL)and Bio-Oss bone powder on alveolar bone remodeling after periodontally accelerated osteogenic orthodontics(PAOO) in rats.Methods Twenty-four 8-week-old male SD rats were selected to establish the PAOO tooth movement model on the palatal side of the right maxillary first molar. They were randomly divided into 3 groups: the corticotomy group(Group A); the corticotomy + Bio-Oss implantation group(Group B); the corticotomy + Bio-Oss + sPL injection group(Group C). On the 7 th, 14 th, 21 st, 28 th, and 56 th days after orthodontic force application, the tooth movement distances in each group were measured respectively, and the movement rates were calculated. At the 4 th and 8 th weeks, 4 rats were randomly selected from Group B and Group C respectively and sacrificed,and maxillary specimens were collected. Micro-CT was used to evaluate the osteogenesis on the palatal side of the right maxillary first molar, and HE staining was used to observe the histomorphological changes.Results There was no significant difference in the tooth movement distances and rates among the three groups of rats(P>0.05). At 4 weeks after the operation, the measured value of BV/TV in Group C was higher than that in Group B(P<0.05), and Tb.Sp was lower than that in Group B(P<0.05). There was no significant difference in BMD, Tb.N,and Tb.Th between the two groups(P>0.05). At 8 weeks after the operation, BV/TV and BMD in Group C were higher than those in Group B, and Tb.Sp was lower than that in Group B, with significant differences(P<0.05).There was no significant difference in Tb. N and Tb. Th between the two groups(P>0.05). Histological staining showed that there was more newly formed bone tissue in Group C than in Group B, and bone remodeling was more active.ConclusionThe combined application of sPL and Bio-Oss bone powder can promote osteogenesis after PAOO in rats.更多还原