【摘要】 目的 探究内质网膜蛋白复合物8(EMC8)在口腔鳞状细胞癌（OSCC）细胞系中的表达及其对OSCC细胞系CAL27、SCC15细胞增殖、迁移、侵袭及凋亡的影响。方法 利用生物信息学技术比较头颈鳞癌组织和正常组织中EMC8的表达差异及EMC8对OSCC的诊断和预后价值的影响。使用免疫印迹（Western Blot）法进一步检测EMC8在OSCC细胞系中的表达水平。将OSCC细胞分别分为Blank组（不做转染处理）、SI-NC组（转染SIRNA-EMC8空表达载体）和SI-EMC8组（转染SIRNA-EMC8沉默载体）。CCK-8法检测EMC8对OSCC细胞增殖能力的影响；细胞划痕实验检测EMC8对OSCC细胞迁移能力的影响；Matrigel Transwell实验检测EMC8对OSCC细胞迁移及侵袭能力的影响；流式细胞术检测EMC8对OSCC细胞凋亡的影响。结果 生物信息学技术分析结果表明，EMC8在头颈鳞癌组织中表达高于正常组织（P<0.01）。WB实验结果表明，与HOK细胞相比，EMC8在CAL27、SCC15细胞中的表达水平升高（P<0.01）。与SI-NC组相比，SI-EMC8口腔增殖、迁移、侵袭能力明显降低（P<0.01），凋亡率上升（P<0.01）。结论 EMC8在OSCC细胞中表达升高，对OSCC具有诊断和预后意义，可以促进OSCC细胞的增殖、迁移、侵袭能力，抑制细胞凋亡。更多还原

【Abstract】 Objective To establish a clinical prognostic model of lnc RNA associated with disulfide death in oralsquamous cell carcinoma(OSCC), aiming to assess patient prognosis.MethodsComparison of EMC8 expressiondifferences between head and neck squamous carcinoma tissues and normal tissues and the effect of EMC8 on thediagnostic and prognostic value of OSCC using bioinformatics techniques. The human oral mucosal keratinizing cellline HOK, and OSCC cell lines CAL27 and SCC15 were cultured in vitro, and the expression levels of EMC8 indifferent cell lines were further detected using immunoblotting(Western Blot) method. OSCC cells were dividedinto Blank group(no transfection treatment), SI-NC group(transfected with SIRNA-EMC8 null expression vector)and SI-EMC8 group(transfected with SIRNA-EMC8 silencing vector), respectively. CCK-8 assay was used todetect the effect of EMC8 on the proliferative ability of OSCC cells; cell scratch assay was used to detect themigratory capacity of OSCC cells. The effect of EMC8 on the migration and invasion ability of OSCC cells wasdetected by Matrigel Transwell assay; the effect of EMC8 on the apoptosis of OSCC cells was detected by flowcytometry.ResultsThe results of bioinformatics technology analysis showed that the expression of EMC8 in head and necksquamous carcinoma tissues was higher than that in normal tissues(P<0.01). The results of WB assay showed that theexpression level of EMC8 was increased in CAL27 and SCC15 cells compared with HOK cells(P<0.01). Compared with theSI-NC group, the proliferation, migration and invasion ability of OSCC cells in the SI-EMC8 group were significantlyreduced(P<0.01), and the apoptosis rate increased(P<0.01).ConclusionElevated expression of EMC8 in OSCCcells has diagnostic and prognostic significance for OSCC, and can promote the proliferation, migration, andinvasive ability of OSCC cells and inhibit apoptosis.更多还原