【摘要】 目的 探讨养血通脉方对利血平诱导低血压大鼠氧化应激及循环肾素-血管紧张素系统(RAS)稳态的影响。方法 取100只雄性Wistar大鼠，随机选取10只作为空白对照组，其余90只大鼠每天2次灌胃利血平悬浊液0.032 mg/kg至实验结束。将连续灌胃利血平3周成功建立低血压模型的50只大鼠随机分为模型对照组、盐酸米多君组及养血通脉低、中、高剂量组，每组10只。盐酸米多君组给予盐酸米多君溶液0.45 mg/(kg·d)灌胃，养血通脉各组分别给予相应剂量养血通脉汤剂灌胃，空白对照组与模型对照组给予纯水10 m L/(kg·d)灌胃。观察干预期间各组大鼠体重、收缩压(SBP)及心率变化，干预4周末检测大鼠血清与脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA)水平及血清肾素(Renin)、血管紧张素转换酶(ACE)、血管紧张素Ⅱ(AngⅡ)水平。结果 与空白对照组比较，模型对照组大鼠体重增长缓慢，SBP降低，心率增快，血清及脑组织中SOD水平均明显降低(P均<0.05)，血清及脑组织中MDA水平和血清Renin、ACE、AngⅡ水平均明显增高(P均<0.05)。干预期间，盐酸米多君组与养血通脉各组大鼠体重增长改善，SBP升高，心率回落，前2周以盐酸米多君组SBP提升幅度最大，后2周养血通脉各组SBP平稳回升，盐酸米多君组稍有回落。干预4周末与模型对照组比较，养血通脉中、高剂量组及盐酸米多君组大鼠体重和各药物组大鼠SBP均明显升高(P均<0.05);各药物组大鼠血清SOD水平和养血通脉中、高剂量组大鼠脑组织中SOD水平均明显升高(P均<0.05)，各药物组大鼠血清MDA、ACE、AngⅡ水平和养血通脉中、高剂量组及盐酸米多君组大鼠脑组织中MDA水平均明显降低(P均<0.05)。结论 养血通脉方可改善利血平诱导低血压大鼠的体重、血压及心率，其较盐酸米多君的升压效应相对缓慢但更为平稳，同时在减轻机体氧化应激、调控循环RAS稳态方面显示出良好的改善效应。更多还原

【Abstract】 Objective It is to explore the effects of decoction for nourishing blood and unblocking pulse on oxidatiue stress and circulatory renin-angiotensin system(RAS) homeostasis in rats with hypotension induced by reserpine. Methods One hundred male Wistar rats were taken,in which 10 rats were randomly selected as the blank control group,and the remaining 90 rats were gavaged with rifampicin suspension 0. 032 mg/kg twice a day until the end of the experiment. The 50 rats that successfully established hypotension model by continuous gavage of rifampicin for 3 weeks were randomly divided into model control group,midodrine hydrochloride group and low,medium and high dose groups of decoction for nourishing blood and unblocking pulse,with 10 rats in each group. The midodrine hydrochloride group was given midodrine hydrochloride solution 0. 45 mg/(kg·d) by gavage,the low,medium and high dose groups of decoction for nourishing blood and unblocking pulse were given decoction for nourishing blood and unblocking pulse of respective dose by gavage,while the blank control group and model control group were given pure water 10 m L/(kg · d) by gavage. The changes in body weight,systolic blood pressure(SBP) and heart rate of the rats during the intervention period were observed,the levels of superoxide dismutase(SOD),malondialdehyde(MDA) in serum and brain tissues,and the levels of renin,angiotensinconverting enzyme(ACE),and angiotensin Ⅱ(Ang Ⅱ) in serum of rats in each group were measured at the end of 4 weeks of intervention. Results Compared with the blank control group,the weight gain of the rats in the model control group was slow,the SBP was decreased,the heart rate was increased,the SOD levels in serum and brain tissue were significantly decreased(all P < 0. 05),the MDA levels in serum and brain tissue,and the levels of Renin,ACE,Ang Ⅱ in serum were significantly increased(all P < 0. 05). During the intervention period,the rats in the midodrine hydrochloride group and each decoction for nourishing blood and unblocking pulse group showed improved body weight gain,increased SBP,and decreased heart rate,with the greatest increase of SBP in the midodrine hydrochloride group in the first 2 weeks,followed by a smooth recovery of SBP in each decoction for nourishing blood and unblocking pulse group in the second 2 weeks,and a slight decrease in the midodrine hydrochloride group. Compared with the model control group at the end of 4 weeks of intervention,the body weight of rats in the middle and high dose groups of decoction for nourishing blood and unblocking pulse and midodrine hydrochloride group,and the SBP of rats in each decoction for nourishing blood and unblocking pulse group were significantly increased(all P < 0. 05); the levels of serum SOD of rats in each treatment group,and the levels of SOD in brain tissues in the middle and high-dose groups of decoction for nourishing blood and unblocking pulse were significantly increased(all P < 0. 05),the levels of serum MDA,ACE and Ang Ⅱ of rats in each treatment group and the levels of MDA in brain tissues of rats in the middle and high dose groups of decoction for nourishing blood and unblocking pulse and midodrine hydrochloride group were all significantly decreased(all P < 0. 05). Conclusion Decoction for nourishing blood and unblocking pulse can improve the body weight,blood pressure and heart rate of rifampicin-induced hypotensive rats,and its blood pressure increasing effect is relatively slower but smoother than that of midodrine hydrochloride,meanwhile,it has good improvement effects in the alleviation of oxidative stress in the body and the regulation of circulating RAS homeostasis.更多还原