【摘要】 该文探讨了对羟基肉桂酸（p-HCA）通过抑制泡沫细胞形成抗动脉粥样硬化的效应。采用氧化型低密度脂蛋白（ox-LDL）构建巨噬细胞源性泡沫细胞模型,通过细胞油红O、Western blot和ELISA实验检测p-HCA对该模型中脂质积累和炎症反应相关指标的影响;采用Apo E^–/–小鼠构建经典的动脉粥样硬化模型,通过主动脉整体油红O、主动脉窦冰冻油红O和免疫组化实验探讨p-HCA体内抗动脉粥样硬化的效应。结果表明, p-HCA通过抑制巨噬细胞脂质吞噬相关蛋白凝集素样氧化型低密度脂蛋白受体-1（LOX-1）和Toll样受体4（TLR4）的表达显著减少了巨噬细胞中脂质的积累;同时p-HCA有效抑制了信号转导与转录激活因子3（STAT3）和白细胞介素6（IL-6）的表达;进一步体内实验结果表明,p-HCA显著减少了Apo E^–/–小鼠主动脉的病变面积,并降低了主动脉中LOX-1、TLR4、STAT3和IL-6的表达水平。总之, p-HCA通过减少巨噬细胞脂质积累和抑制炎症发展发挥了抗动脉粥样硬化的效应。更多还原

【Abstract】 This study investigated the anti-atherosclerotic effect of p-HCA（p-hydroxycinnamic acid） by inhibiting foam cell formation. ox-LDL（oxidised low-density lipoprotein） was used to construct a macrophage-derived foam cell model, and the effects of p-HCA on lipid accumulation and inflammatory response related indicators in this model were detected by cellular oil red O, Western blot and ELISA experiments. ApoE^–/– mice were used to build a classical atherosclerosis model, and the anti-atherosclerotic effect of p-HCA in vivo was explored by aortic whole oil red O, aortic sinus frozen oil red O and immunohistochemistry assays. The results showed that p-HCA significantly reduced lipid accumulation in macrophages by inhibiting the expression of macrophage lipid phagocytosis-associated proteins lectin-like oxidised LOX-1（lectin-like oxidized low-density lipoprotein receptor 1） and TLR4（toll-like receptor 4）, meanwhile, p-HCA effectively constrained STAT3（signal transducer and activator of transcription 3） and IL-6（interleukin-6） expression. Further in vivo experiments showed that p-HCA reduced the lesion area in the aorta of Apo E–/– mice and decreased the expression of LOX-1, TLR4, STAT3 and IL-6 in the aorta. In conclusion, p-HCA exerts an anti-atherosclerotic effect by reducing macrophage lipid accumulation and inflammatory response.更多还原