【摘要】 准确区分S^2-与HS^-对于环境监测、病理调节和生物医药研究至关重要.以氧杂蒽衍生物与香豆素衍生物构建FRET型荧光团，再分别选用链状酰氯（己酰氯）与环状酰氯（环己酰氯）作为识别基团，合成了两例FRET型荧光探针.采用荧光光谱技术对其检测性能进行了探索与分析.结果表明，该探针对S^2-有特异性识别，随着S^2-浓度的增大，460 nm处的荧光减弱，540 nm处的荧光增强；当S^2-浓度分别达到1 760μmol/L和800μmol/L时两个探针的荧光强度达到饱和，且在该范围内呈现良好的线性关系，检测限为2.73μmol/L和0.71μmol/L.这种探针为特异性识别S^2-提供了一种新的策略，在水质、生理检测S^2-方向的应用具有一定潜力.更多还原

【Abstract】 Accurately distinguishing S^2- from HS^- is crucial for environmental monitoring, pathological regulation, and biomedical research. FRET type fluorescent probes were constructed using anthraquinone derivatives and coumarin derivatives, and two FRET type fluorescent probes were synthesized using chain acyl chloride（hexanoyl chloride） and cyclic acyl chloride（cyclohexanol chloride） as recognition groups, respectively. The detection performance was explored and analyzed using fluorescence spectroscopy technology. The results indicate that the probe has specific recognition for S^2-, and as the concentration of S^2- increases, the fluorescence at 460 nm decreases and the fluorescence at 540 nm increases; When the S^2- concentration reached 1 760 μmol/L and 800 μmol/L respectively, the fluorescence intensity of the two probes reached saturation and showed a good linear relationship within this range. The detection limits were 2.73 μmol/L and 0.71 μmol/L. This probe provides a new strategy for specific recognition of S^2- and has certain potential for applications in water quality and physiological detection of S^2- direction.更多还原