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9种中药渣对秸秆体外发酵及甲烷生成的影响

Effects of Nine Types of Herbal Residues on In Vitro Fermentation and Methane Production of Straw

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【作者】 郭冬生; 唐武; 贺志雄; 汤少勋;

【Author】 GUO Dongsheng;TANG Wu;HE Zhixiong;TANG Shaoxun;College of Life and Environmental Science, Zoology Key Laboratory of Hunan Higher Education, Hunan University of Arts and Science;State Key Laboratory of Forage Breeding-by-Design and Utilization,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, and Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences;College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences;Yuelushan Laboratory;

【通讯作者】 汤少勋;

【机构】 湖南文理学院生命与环境科学学院,动物学湖南省高校重点实验室; 饲草种质高效设计与利用国家重点实验室,畜禽养殖污染控制与资源化技术国家工程实验室,湖南省动物营养生理与代谢过程重点实验室,中国科学院亚热带农业生态研究所; 中国科学院大学现代农业科学学院; 岳麓山实验室;

【摘要】 为研究不同中药渣对秸秆发酵特性及温室气体排放的影响,以3只装有瘤胃瘘管的成年湘东黑山羊作为瘤胃液供体动物,设计9×2+1两因素完全随机试验,将9种中药渣(白术、柴胡、甘草、黄柏、黄连、黄芪、肉桂皮、山银花和玄参)分别按0.5%和1.0%的水平添加到玉米秸秆中,测定产气量、瘤胃发酵参数、微生物蛋白产量MCP、代谢能ME以及CH4和H2的产量。结果表明,相对对照组,添加白术、黄连和肉桂皮可显著提高秸秆理论最大产气量(Vf;P<0.0001),添加肉桂皮显著提高起始降解速率(FRD0)(P<0.05)。添加中药渣显著降低发酵液pH(P<0.05),添加1.0%水平中药渣的pH和NH3-N浓度显著高于0.5%水平(P<0.05)。黄连处理显著提高NH3-N浓度(P<0.05),白术和黄连组的ME和MCP产量显著增加(P<0.05)。添加白术、黄连、黄芪、肉桂皮和山银花相比对照组显著提高了CH4和H2的Vf(P<0.0001),9种中药渣中白术、黄连、黄芪和肉桂皮的CH4-Vf值显著高于玄参、黄柏和柴胡(P<0.05),H2-Vf值显著高于山银花(P<0.05)。黄芪、白术和肉桂皮组的CH4产气分率(k)显著高于对照组(P<0.05)。综上所述,白术和黄连在本研究添加水平下可促进玉米秸秆的发酵效率及MCP合成,但可能不利于甲烷减排。

【Abstract】 In order to investigate the effects of different herbal residues on straw fermentation characteristics and greenhouse gas emissions,a 9×2+1 two-factor completely randomized design was implemented using three adult Xiangdong black goats fitted with rumen fistulas as rumen fluid donors.Nine typesof herbal residues(Atractylodes macrocephala,AMac;Bupleuri Radix,BR; Glycyrrhiza uralensis Fisch.,GUF;Phellodendron amurense Rupr,,PA;Coptidis Rhizoma,CR;Astragalus membranaceus,AMem;Cinnamomum cassia,CC;Lonicerae flos,LF;and Scrophularia ningpoensis Hemsl.,SNH) were added to corn stover at 0.5% and 1.0% levels.Parameters measured included gas production,rumen fermentation parameters,microbial crude protein(MCP) yield,metabolizable energy(ME),and generation of CH4 and H2.The results demonstrated that,compared to the control group,supplementation with AMac,CR and CC significantly increased the theoretical maximum gas production(Vf;P<0.000 1).CC also elevated the initial degradation rate(FRD0;P<0.05).Herbal residue addition significantly reduced fermentation liquid pH value(P<0.05),with 1.0% treatments showing higher pH and NH3-N concentrations than 0.5% treatments(P<0.05).CR treatment increased NH3-N concentration(P <0.05),while AMac and CR groups exhibited significantly higher ME and MCP yields(P<0.05).Supplementation with AMac,CR,AMem,CC,and LF significantly enhanced the Vf of CH4 and H2 relative to the control(P<0.000 1).Among the nine herbs,AMac,CR,AMem,and CC displayed higher CH4-Vf values than SNH,PA,and BR(P<0.05),and their H2-Vf values surpassed those of LF(P<0.05).The CH4 production rate constant(k) in AMem,AMac,and CC groups was significantly higher than that in the control(P<0.05).In conclusion,at the tested inclusion levels,AMac and CR enhance fermentation efficiency and MCP synthesis in corn stover but may not contribute to methane mitigation.

【基金】 内蒙古揭榜挂帅项目(2022JBGS0023);国家重点研发计划项目(2023YFD1301701);湖南省现代农业产业技术草食动物产业技术体系项目~~
  • 【文献出处】 西北农业学报 ,Acta Agriculturae Boreali-occidentalis Sinica , 编辑部邮箱 ,2025年10期
  • 【分类号】S816.7
  • 【下载频次】76
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