【摘要】 以霍山石斛为材料，基于非靶向代谢组技术研究3种栽培模式(贴石栽培、林下栽培和大棚栽培)和两种生长年限(3 a和4 a)对其茎干代谢物成分差异的影响。结果表明：共鉴定到3 181个代谢物，属于库中、KEGG和HMDB代谢物分别有2 865个、1 362个和2 851个。HMDB化合物3级分类中排在前3的分别为氨基酸、多肽和类似物(12.03%)、碳水化合物和碳水化合物结合物(9.82%)、黄酮苷(4.38%)。KEGG化合物分类中位列前3的分别是氨基酸类、磷脂类和单糖类。不同栽培模式间或不同年限间均具有差异显著代谢物，其中最高是L4 vs S3之间有771个，最低的是P4 vs P3之间只有54个。Venn图分析表明本研究共有的代谢物为2 806个，且每处理都有自己特有的代谢物；差异代谢物Venn图分析显示每处理都有自己特有的显著差异代谢物，但无共同的。总之，栽培模式和生长年限均会影响霍山石斛代谢物成分的组成和含量，贴石栽培和林下栽培比大棚栽培模式影响更为明显，4 a生长年限比3 a特有代谢物更丰富。更多还原

【Abstract】 To investigate the differences in metabolites of Dendrobium huoshanense stems cultivated under three different cultivation modes(sticking on stone cultivation, under forest cultivation and greenhouse cultivation) and its growth years( 3-and 4-year),untargeted metabolomics techniques were employed.The results showed that a total of 3 181 metabolites were identified, with 2 865,1 362 and 2 851 metabolites assigned to the library, KEGG and HMDB,respectively.In the third level classification of HMDB,the top three compounds were amino acids, peptides, and analogs(12.03%); carbohydrates and carbohydrate conjugates(9.82%); and flavonoid glycosides(4.38%).For KEGG classification, the leading three categories were amino acids, phospholipids, and monosaccharides.Significant differences in metabolites were observed among different cultivation modes and growth years, with the highest number of differences(771) between L4 and S3,and the lowest(54) between P4 and P3.Venn diagram analysis showed that there were 2 806 common metabolites across different cultivation modes and growth years, and each treatment owned unique metabolites.However, there were no shared significant differential metabolites across different cultivation modes and growth years, and unique significant differences in metabolites existed among them.Overall, the composition and content of metabolites in D.huoshanense are significantly impacted by both cultivation modes and growth years, stone attachment cultivation under forest cultivation has more pronounced effects compared to greenhouse cultivation, and 4-year samples contain a wider array of unique metabolites.更多还原