【摘要】 旨在对国家二级保护野生动物多鳞白甲鱼遗传多样性进行评价。采用限制性内切位点相关DNA测序(restriction site-associated DNA sequencing, RAD-seq))技术，从秦岭南北坡两个种群中鉴定出425205个全基因组高置信度群体单核苷酸多态性(single nucleotide polymorphisms, SNPs)。秦岭北坡多鳞白甲鱼的期望杂合数、核苷酸多样性和多态性信息含量分别为0.177、0.200和0.145,而秦岭南坡多鳞白甲鱼的期望杂合数、核苷酸多样性和多态性信息含量分别为0.261、0.291和0.212。种群结构分析结果表明，秦岭南北坡两个多鳞白甲鱼地理种群的遗传多样性存在明显差异。更多还原

【Abstract】 The aim of this studyis to evaluate the genetic diversity of Onychostoma macrolepis,a species of national secondary protected wildlife.In this study, restriction site-associated DNA sequencing(RAD-seq) was used to identify 425 205 genome-wide high-confidence population single nucleotide polymorphisms(SNPs) across two populations on the northern and southern slopes of the Qinling Mountains.The expected heterozygosity, nucleotide diversity and polymorphism information content of O.macrolepis on the north slope of the Qinling Mountains were 0.177,0.200 and 0.145,respectively, while these three parameters of O.macrolepis on the south slope of the Qinling Mountains were 0.261,0.291 and 0.212,respectively.The population structure analysis revealed a clear contrast in genetic diversity between the two geographical populations.更多还原