【摘要】 目的：分析肺炎支原体（MP）感染患儿23S rRNA基因位点突变情况及其与咽拭子MP-DNA载量、临床症状的相关性。方法：回顾性分析2024年3月1日—2025年6月1日克拉玛依市中心医院收治的150例MP感染患儿的临床资料。根据23S rRNA基因位点突变情况将患儿分为突变组（n=88）和未突变组（n=62），比较两组患儿临床资料，分析23S rRNA基因位点突变与咽拭子MP-DNA载量及临床症状的相关性。结果：150例MP感染患儿中，88例患儿发生23S rRNA基因位点突变，其中85例为2063位点突变，3例为2064位点突变。两组白细胞计数、C反应蛋白（CRP）、乳酸脱氢酶、降钙素原、MP-DNA载量、肺实变、重症肺炎支原体肺炎（MPP）情况与发热及住院时间比较，差异有统计学意义（P<0.05）。Spearman相关性分析结果显示，23S rRNA基因位点突变与CRP水平、MP-DNA载量、重症MPP、发热时间及住院时间呈正相关（P<0.001）。结论：MP感染患儿易发生23S rRNA基因位点突变，以2063位点突变居多，23S rRNA基因位点突变与患儿CRP水平、MPDNA载量、重症MPP、发热时间及住院时间相关。更多还原

【Abstract】 Objective: To analyze the mutation status of the 23S rRNA gene locus in children with Mycoplasma pneumoniae(MP)infection and its correlation with the MP-DNA load in throat swabs and clinical symptoms. Methods: A retrospective analysis was conducted on the clinical data of one hundred and fifty children with MP infection admitted to Karamay Central Hospital from March 1, 2024 to June 1, 2025. According to the mutation status of the 23S rRNA gene locus, the children were divided into the mutation group(n=88) and the non-mutation group(n=62). The clinical data of the two groups of children were compared to analyze the correlation between the 23S rRNA gene locus mutation and the MP-DNA load in throat swabs as well as clinical symptoms. Results: Among 150 children with MP infection, 88 children had mutations at the 23S rRNA gene locus, among which85 cases had mutations at the 2063 locus and 3 cases had mutations at the 2064 locus. There were statistically significant differences in white blood cell count, C-reactive protein(CRP), lactate dehydrogenase, procalcitonin, MP-DNA load, lung consolidation, severe mycoplasma pneumoniae pneumonia(MPP), fever and hospital stay between the two groups(P<0.05). The results of Spearman correlation analysis showed that the mutation of the 23S rRNA gene locus was positively correlated with CRP level, MP-DNA load, severe MPP, fever duration and hospital stay(P<0.001). Conclusion: Children infected with MP are prone to mutations at the 23S rRNA gene locus, with mutations at the 2063 locus being the most common. Mutations at the 23S rRNA gene locus are related to the CRP level, MP-DNA load, severe MPP, fever duration and hospital stay of the children.更多还原