【摘要】 为探索调控超甜玉米种子发芽率的显著关联位点及候选基因，对100份超甜玉米自交系种子发芽率相关性状进行测定，并利用超甜玉米56K基因芯片，采用MLM(Q+K)模型，通过GWAS分析，共关联定位到5个与超甜玉米种子发芽率有显著关联的SNP位点，并在上下游200 kb的置信区间内，发现了43个已有功能注释基因，基于文献比较得出Zm00001eb057130、Zm00001eb057170、Zm00001eb057030、Zm00001eb145440、Zm00001eb145480、Zm00001eb057150、Zm00001eb145520、Zm00001eb057110这8个基因是调控超甜玉米种子发芽率的候选基因，其中有5个候选基因在种子的胚胎发育中发挥作用，从而直接影响种子的发芽率，有3个候选基因主要通过参与种子的胚根生长发育间接影响种子的发芽率。本研究结果可为选育高发芽率的超甜玉米品种提供理论支撑，为精细定位和克隆发芽率相关性状基因提供参考。更多还原

【Abstract】 The germination rate-related characters of 100 inbred lines of super sweet corn were measured to explore the significant associated loci and candidate genes regulating the germination rate of super sweet corn seeds. Using the super sweet corn 56K gene chip,the MLM(Q + K) model was adopted to locate a total of 5 SNPs correlated with the germination rate of super sweet corn seeds through GWAS analysis,and 43 functionally annotated genes were found within the upper and lower 200 kb confidence interval. Based on literature comparison, 8 candidate genes(Zm00001eb057130, Zm00001eb057170,Zm00001eb057030,Zm00001eb145440,Zm00001eb145480,Zm00001eb057150,Zm0000-1eb145520, Zm00001eb057110) were found to be related to the regulation of seed germination rate of super sweet corn. Among them,5 candidate genes played a role in the embryonic development of seeds,thus directly affecting the germination rate of seeds,and 3candidate genes indirectly affected the germination rate of seeds,mainly by participating in the root growth and development of seeds. The results of this study can provide theoretical support for breeding super sweet corn varieties with high germination rates,which provides a reference for fine mapping and cloning of genes related to germination rate.更多还原