【摘要】 针对传统液相色谱教学中理论与实践割裂、学生被动操作仪器的问题，提出以“色谱柱自主制备”为核心的一体化教学模式。不再按照传统的从基础理论到实际应用的顺序，而是从实际操作中遇到的问题出发，逆向引导学生去探寻理论知识，加深对知识的理解。通过引导学生完成色谱柱填装、参数优化及分离实验的全流程实践，结合理论模块的逆向重构，实现知识内化与实践能力培养的双重目标，让学生不仅能够扎实掌握液相色谱的相关知识，还能切实提升自身的实践操作能力。更多还原

【Abstract】 In response to the issues of disconnection between theory and practice and passive instrument operation in traditional liquid chromatography teaching, an integrated teaching model centered on "student-led preparation of chromatographic columns" is proposed. By guiding students through the entire process of column packing, parameter optimization, and separation experiments, and by incorporating reverse-engineered theoretical modules, this approach achieves the dual objectives of knowledge internalization and fostering innovation skills.更多还原