【摘要】 为分离筛选盐碱地适生的大豆根瘤菌，从山东省东营市黄河三角洲农业高新技术产业示范区中、重度盐碱地野大豆及主要栽培大豆品种的根瘤中分离共生根瘤菌，鉴定其分类地位，并评价其结瘤能力及促生功能。采用选择性培养基，从不同大豆及野大豆根瘤样品中共分离获得84株纯培养物，分属3个属6个种，其中96%为剑菌属（Ensifer）。功能评价结果表明，所有菌株均能够产吲哚-3-乙酸（IAA）,32株可耐受2%NaCl,14株具有产铁载体能力，7株能矿化有机磷，14株能溶解无机磷，6株产胞外多糖达200 mg/L以上。水培试验结果表明，菌株63-2-1-1、306-2-1-1和63-2-19-1明显缓解了高盐对大豆幼苗胁迫作用。以上研究结果为开发大豆耐盐根瘤菌菌剂提供菌种资源，对盐碱地大豆适生增产具有重要的实践价值。更多还原

【Abstract】 To isolate and screen soybean rhizobia suitable for promoting growth,soybean symbiotic rhizobia were isolated and screened from the wild and major cultivated soybean varieties in moderate to severe saline-alkali soil of the Yellow River Delta agricultural hightech industrial demonstration zone,Dongying City,Shandong Province. Their nodulation and growth promoting functional characteristics were evaluated. A total of 84 pure cultures were isolated from the nodule samples of soybean,which belong to three genera and six species,and 96% of them belong to Ensifer. The results of functional testing showed that all of them can produce indole-3-acetic acid(IAA),32 strains could tolerate 2% NaCl,14 strains could produce iron carriers,7 strains could mineralize organic phosphorus,14strains could dissolve inorganic phosphorus,and 6 strains could produce organophosphorus of over 200 mg/L. The results of hydroponic experiments showed that strains 63-2-1-1,306-2-1-1,and 63-2-19-1 could significantly alleviate the degree of salt damage to soybean seedlings. This study not only provided strain resources for the development of salt-tolerant microbial agents for soybeans in salinealkali soil but also had important theoretical and practical significance for the growth and yield increase of soybeans in saline-alkali soil.更多还原