【摘要】 目的：探究α-淀粉酶和D-精氨酸联合应用对牙周致病菌及混合生物膜的分散和抑制作用。方法：通过结晶紫染色分别测定α-淀粉酶和D-精氨酸对牙周主要致病菌（戈登链球菌、具核梭杆菌和牙龈卟啉单胞菌）的最小生物膜清除浓度（MBEC）和最小生物膜抑制浓度（MBIC）。通过微量棋盘稀释法确定与0.2%氯己定作用等效的联合用药浓度，并结合激光共聚焦显微镜（CLSM）评估其对多菌种生物膜的分散与抑制效果。结果：α-淀粉酶对单菌种和多菌种生物膜的MBEC、MBIC均为10 g/L。D-精氨酸对单菌种MBEC和MBIC均为128 mmol/L，对多菌种生物膜MBEC为128 mmol/L,MBIC为256 mmol/L。联合应用1.0 g/Lα-淀粉酶和32 mmol/L D-精氨酸的分散作用以及0.5 g/L α-淀粉酶和32 mmol/L D-精氨酸的抑制作用，均与0.2%氯己定效果相当。在CLSM检测的分散和抑制研究中，与单独用药相比，联合应用α-淀粉酶和D-精氨酸处理后的样本中活菌量最少（F=781.3、362.5,P<0.001），且与0.2%氯己定组相比无统计学差异（P>0.05）。结论：α-淀粉酶联合D-精氨酸可显著提高对牙周致病菌混合生物膜的分散和抑制能力。更多还原

【Abstract】 Objective:To evaluate the dispersion and inhibition effect of α-amylase in combination with D-arginine on periodontal pathogens and multispecies biofilms. Methods:Minimum biofilm clearance concentration(MBEC) and minimum biofilm inhibitory concentration(MBIC)of α-amylase and D-arginine against the major periodontal pathogens(Streptococcus gordonii,Fusobacteriumnucleatum,and Porphyromonas gingivalis)were determined by crystalline violet staining. The micro checkerboard dilution method was applied to determine the concentration of drug combination that was equivalent to the effect of 0.2% chlorhexidine(CHX). Combined with confocal laser scanning microscopelaser(CLSM),its dispersion and inhibition effect on multispecies biofilms was assessed.Results:The MBEC and MBIC of α-amylase were 10 g/L for both single-species biofilm and multispecies biofilm. D-Arginine had MBEC and MBIC values of 128 mmol/L for single-species biofilm. D-Arginine had MBEC values of 128 mmol/L,and MBIC values of 256 mmol/L for multispecies biofilm. The dispersion effect of the combined application of 1.0 g/L α-amylase with 32 mmol/L Darginine and the inhibition effect of 0.5 g/L α-amylase with 32 mmol/L D-arginine were comparable to the effect of 0.2% CHX. In the dispersion and inhibition study of CLSM assay,the combined application of α-amylase and D-arginine resulted in the lowest viable bacterial count compared to either agent alone(F=781.3,362.5,P<0.001),with no statistically significant difference from the0.2% CHX group(P>0.05). Conclusion:The combination of α-amylase and D-arginine significantly enhances the dispersion and inhibition efficacy against multispecies biofilms of periodontal pathogens.更多还原