【摘要】 氨基酸透性酶（Amino acid permeases,AAPs）在植物转运氨基酸过程中发挥重要的调节作用，为进一步探究玉米AAP基因（ZmAPP）相关生物学功能，本研究以玉米‘B73’为材料，克隆AAP基因（ZmAPP）对其序列进行生物信息学分析，构建绿色荧光蛋白EGFP表达载体pCAMBIA1300-ZmAAP-EGFP，通过农杆菌介导法转化洋葱表皮，使用荧光显微镜观察基因表达情况。结果表明，玉米AAP基因c DNA序列全长为1 407 bp，编码468个氨基酸，理论等电点（pI）为8.72，不稳定系数为36.93(<40)，总平均亲水性为0.487，属于碱性稳定疏水蛋白。系统进化分析表明，ZmAAP与大麦AAP基因在氨基酸水平序列亲缘关系最近。亚细胞定位结果表明，ZmAAP定位在细胞膜。本研究为后续深入研究玉米AAP基因功能以及氨基酸转运机制提供了一定的研究基础。更多还原

【Abstract】 Amino acid permeases(AAPs)play an important regulatory role in the transport of amino acids in plants. In order to further explore the biological function of maize AAP gene, in this study, the AAP gene(ZmAPP)was cloned from corn ‘B73’,and its sequence was analyzed by bioinformatics. The green fluorescent protein EGFP expression vector PCAMBIA1300-ZmAAP-EGFP was constructed. The onion epidermis was transformed by agrobacterium mediated method, and gene expression was observed by using fluorescence microscopy. The results showed thatthe total length of maize AAP gene cDNA sequence was 1407 bp, encoding 468 amino acids, the theoretical isoelectric point(pI)was 8.72, the instability coefficient was 36.93(<40),and the total average hydrophilicity was 0.487, which belonged to the alkaline stable hydrophobic protein. Multiple sequence alignment and phylogenetic analysis showed that ZmAAP and barley AAP had the highest similarity at amino acid level, and subcellular localization results showed that ZmAAP was localized in cell membrane. This study serves as a basis for further research on maize AAP gene function and amino acid transport mechanism.更多还原