【摘要】 目的 探究定植元对九联益生菌在肠黏膜上皮细胞黏附的影响，以及九联益生菌联合定植元对肠道功能紊乱的疗效。方法 选取20例肠道功能紊乱患者，根据九联益生菌与肠黏膜上皮细胞共孵育时是否联合定植元，分为实验组(九联益生菌菌液联合定植元与肠黏膜上皮细胞共孵育)和对照组(仅以九联益生菌菌液与肠黏膜上皮细胞共孵育),采用自身对照的方式进行观察。分别于共孵育4、12 h后提取出黏附于肠黏膜上皮细胞的九联益生菌，统计形成的菌落数，分析定植元对益生菌在肠黏膜上皮细胞黏附的促进作用。根据胃肠道症状评定量表(gastrointestinal symptom rating scale, GSRS)评分，对两组的治疗效果进行评估。结果 共孵育4 h后，从实验组提取出的黏附于细胞上的九联益生菌在培养24 h后形成的菌落多于对照组(P<0.05);共孵育12 h后，再培养的菌落显著多于对照组(P<0.01)。经联合治疗，患者GSRS评分较治疗前显著降低(P<0.01),腹痛、腹泻等症状明显改善。结论 定植元对九联益生菌在肠黏膜上皮细胞上的黏附有促进作用。益生菌联合定植元可能成为治疗肠道功能紊乱的有效方法之一。更多还原

【Abstract】 Objective To explore the influence of colonization factor(CF) on the adhesion of Jiulian probiotics to intestinal mucosal epithelial cells, and the efficacy of Jiulian probiotics combined with CF in treating intestinal dysfunction. Methods Twenty patients with intestinal dysfunction were selected. Based on whether the Jiulian probiotics were co-cultured with intestinal mucosal epithelial cells in combination with CF, they were divided into an experimental group(the Jiulian probiotic suspension was co-cultured with intestinal mucosal epithelial cells in combination with CF) and a control group(only the Jiulian probiotic suspension was co-cultured with intestinal mucosal epithelial cells). Observation was conducted using a self-controlled approach. The Jiulian probiotics that adhered to the intestinal mucosal epithelial cells were extracted separately 4 and 12 hours after co-cultivation; the number of formed colonies was counted, and the effect of CF on promoting the adhesion of the probiotics to the intestinal mucosal epithelial cells was analyzed. According to the gastrointestinal symptom rating scale(GSRS) scores, the curative effect was assessed between the two groups. Results The Jiulian probiotics adherent to cells, harvested from the experimental group after 4 hours of co-cultivation, formed significantly more colonies than those harvested from the control group after 24-hour of cultivation(P<0.05), while the formed colonies after 12 hours of co-cultivation were significantly more than those harvested from the control group(P<0.01). After the combination treatment, the GSRS score of the patients was significantly lower than that before the treatment(P<0.01), while symptoms such as abdominal pain and diarrhea were significantly improved. Conclusion CF could promote the adhesion of Jiulian probiotics to intestinal mucosal epithelial cells. Probiotics combined with CF may become one of effective methods for treating intestinal dysfunction.更多还原