【摘要】 目的 探讨C57BL/6J小鼠手术胚胎移植的最佳条件，分析胚胎左侧、右侧、双侧移植及胚胎数目对移植成功率的影响。方法 将C57BL/6J的新鲜胚胎转移到受体ICR雌鼠输卵管，经过夜培养至2细胞阶段，检测左侧、右侧及双侧分别移植15枚、20枚和25枚胚胎时受体鼠妊娠率、窝均产仔数和胚胎存活率。结果 移植类型和胚胎数目对移植胚胎的存活率均有显著影响。双侧移植和右侧移植的存活率高于左侧移植(P<0.05)。移植20枚胚胎时，右侧移植和双侧移植有较高的产仔数和胚胎存活率(P<0.05)。双侧移植25枚胚胎时，窝均产仔数最多，但胚胎存活率与双侧、右侧移植20枚胚胎时无显著差异。结论 C57BL/6J小鼠使用20枚胚胎进行右侧输卵管移植具有更好的移植效果，且因单侧移植手术切口小，可减少手术时间、降低术后疼痛，改善胚胎移植过程的动物福利。更多还原

【Abstract】 Objective To investigate the best conditions for surgical embryo transfer in C57BL/6J mice by evaluating the effects of embryo number and transfer location(unilateral or bilateral) on success rates.Methods C57BL/6J embryos were cultured overnight to the two-cell stage and then transferred into ICR female mice. Researchers examined pregnancy rates, average litter size, and embryo survival under different conditions, including left, right, or bilateral surgeries with 15, 20, or 25 embryos transplanted.Results Transplantation type and embryo number significantly affected embryo survival rates. Bilateral and right-sided transplants had higher survival rates than left-sided ones(P<0.05). Transplanting 20 embryos to the right side or bilaterally increased the number of pups and survival rate(P<0.05). Transplanting 25 embryos bilaterally resulted in the highest average number of pups, but the survival rate was similar to 20 embryos transplanted bilaterally or to the right side.Conclusion Right-sided oviductal transplantation of 20 embryos in C57BL/6J mice not only yields superior transplantation outcomes but also minimizes surgical incisions and post-operative pain, enhancing animal welfare during the procedure.更多还原