【摘要】 目的:探讨山奈酚调节Hippo信号通路对脊髓星形胶质细胞氧化损伤的影响。方法:将大鼠脊髓星形胶质细胞分为：对照组、氧化损伤组、L-山奈酚组、H-山奈酚组、H-山奈酚+抑制剂组，除对照组外，其余组均采用200μmol/L过氧化氢（H₂O₂）培养24 h以诱导氧化损伤，MTT法检测细胞活力；TUNEL染色检测凋亡；试剂盒检测脂质氧化（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）水平；活性氧荧光探针测定活性氧水平；Western blot检测脊髓星形胶质细胞中Yes相关蛋白1（YAP）蛋白表达。结果:与对照组比较，氧化损伤组细胞活力降低，TUNEL阳性细胞率升高，GSH-Px、SOD的水平降低，MDA和活性氧水平提高，p-YAP蛋白表达降低（均P<0.05）;与氧化损伤组比较，L-山奈酚组、H-山奈酚组细胞活力提高，TUNEL阳性细胞率降低，GSH-Px、SOD的水平提高，MDA和活性氧水平降低，p-YAP蛋白表达提高（均P<0.05）,H-山奈酚组脊髓星形胶质细胞指标变化大于L-山奈酚组（P<0.05）;YAP抑制剂抑制脊髓星形胶质细胞中p-YAP蛋白表达，脊髓星形胶质细胞的氧化损伤加重（P<0.05）。结论:山奈酚调节Hippo信号通路减轻脊髓星形胶质细胞氧化损伤。更多还原

【Abstract】 Objective:To explore the effect of kaempferol on oxidative damage of spinal cord astrocytes by regulating the Hippo signaling pathway.Methods:Rat spinal cord astrocytes were assigned into control group, oxidative damage group, L-kaempferol group, H-kaempferol group, and H-kaempferol+inhibitor group.Except for the control group, all other groups were cultured with 200 μmol/L H₂O₂ for 24 hours to induce oxidative damage.MTT assay was performed to detect cell viability.TUNEL staining was used to detect apoptosis.The reagent kits were used to detect the malondialdehyde（MDA）,superoxide dismutase（SOD）,and glutathione peroxidase（GSH-Px）.Reactive oxygen species fluorescent probe was used to measure reactive oxygen species.Western blot was performed to detect the Yes associated protein 1（YAP） protein in spinal cord astrocytes.Results:Compared with the control group, the oxidative damage group showed a decrease in cell viability, an increase in TUNEL positive cell rate, a decrease in GSH-Px and SOD,an increase in MDA and reactive oxygen species, and a decrease in p-YAP protein（all P<0.05）.Compared with the oxidative damage group, the L-kaempferol group and H-kaempferol group showed increased cell viability, decreased TUNEL positive cell rate, increased GSH-Px and SOD,decreased MDA and reactive oxygen species, and increased p-YAP protein（all P<0.05）,the changes in spinal cord astrocytes in the H-kaempferol group were greater than those in the L-kaempferol group（P<0.05）.YAP inhibitor inhibited the p-YAP protein in spinal astrocytes, exacerbated oxidative damage in spinal astrocytes（P<0.05）.Conclusion:Kaempferol regulates the Hippo signaling pathway to alleviate oxidative damage in spinal cord astrocytes.更多还原