【摘要】 【目的】研究果蔗‘Badila’中甘蔗线条花叶病毒（sugarcane streak mosaic virus, SCSMV）的高效脱毒技术，为提升果蔗产量提供技术支撑。【方法】以携带SCSMV的‘Badila’为试验材料，设置未处理对照、50℃2 h、50℃3 h、52℃3 h、54℃3 h和58℃1 h共6个温水处理组，结合茎尖组织培养和芽尖组织培养生产脱毒苗，并对脱毒苗进行SCSMV病原分子检测，同时调查脱毒苗桶栽后的农艺性状，综合分析不同处理对SCSMV的脱除效果。【结果】温水处理显著降低蔗芽出芽率，且温度和处理时间与出芽率呈负相关。当温度升至54℃和58℃时，蔗芽完全丧失萌发能力，导致无法获取茎尖组织培养材料；然而，该处理对芽分生组织培养的成活率影响较小，即使在54℃和58℃条件下，芽尖分生组织仍保持较高的成活率（83.3%和76.9%）。在病毒脱除效果方面，50℃2 h、50℃3 h、52℃3 h温水处理结合茎尖分生组织培养仅能降低SCSMV检出率至80%，而相同条件下芽尖培养则分别降至70%、60%和30%。特别是在58℃处理1 h条件下，芽尖培养的病毒脱除率可达100%。田间试验表明，脱毒处理的甘蔗植株在长势和农艺性状方面均显著优于对照，其中，株高、中部茎节间长度、单茎重、锤度和蔗糖分分别提高43.5%、53.5%、58.3%、12.4%和30.0%。【结论】成功建立了温水处理结合芽尖组织培养的SCSMV脱除技术体系，该技术具有成活率高、脱毒效率高等优点。更多还原

【Abstract】 【Objective】 This study is aimed to investigate efficient virus elimination technology for Sugarcane Streak Mosaic Virus(SCSMV) in chewing cane ‘Badila’, providing technical support for improving sugarcane yield.【Method】Using SCSMV-infected ‘Badila’ as experimental material, six hot water treatment groups were established: Untreated control, 50 ℃ for 2 h, 50 ℃ for 3 h, 52 ℃ for 3 h, 54 ℃ for 3 h, and 58 ℃ for 1 h. Virus-free plantlets were produced through shoot-tip culture and bud-tip culture. The virus-free plantlets were subjected to molecular detection of SCSMV, and their agronomic traits were investigated after pot cultivation to comprehensively analyze the virus elimination effects. 【Result】 Hot water treatment significantly reduced bud germination rates, with temperature and treatment duration showing a negative correlation with germination rate. No sprouting was observed at 54 ℃ and 58 ℃, making it impossible to obtain shoot-tip explants for tissue culture. However, the treatment had minimal impact on the survival rate of bud-tip culture. Even at 54 ℃ and 58 ℃, the bud apical meristems still maintained relatively high survival rates(83.3% and 76.9%, respectively). In terms of virus elimination, hot water treatment at 50 ℃ for 2 h, 50 ℃ for 3 h, and 52 ℃ for 3 h combined with shoot-tip culture only reduced the SCSMV detection rate to 80%. Under the same conditions, bud-tip culture reduced the rates to 70%, 60%, and 30%, respectively. Notably, under 58 ℃ treatment for 1 h, budtip culture achieved complete virus elimination(100%). Field trials demonstrated that virus-free sugarcane plants exhibited significantly better growth and agronomic traits compared to the control group. Plant height, internode length, single stem weight, brix and sugar content increased by 43.5%, 53.5%, 58.3%, 12.4% and 30.0%, respectively. 【Conclusion】 This study successfully established a virus elimination system combining hot water treatment and bud-tip culture, characterized by high plantlet regeneration and virus elimination efficiency.更多还原