【摘要】 为改善血红素(Heme)在中性条件下的水溶性，首先将猪血红蛋白(Porcine hemoglobin,PHb)酸水解制得不溶性Heme-肽，再以乳清蛋白(Whey protein,WP)和水解得到的Heme为原料，构建WP-Heme纳米复合物，从而显著改善Heme的水溶性和生物利用率。结果表明：在85 ℃、pH2.0条件下，PHb水解12 h，水解度达到40%以上，得到含16种氨基酸的Heme-肽水解混合物，其中Heme含量为5%；Heme在中性条件下与WP相互作用，制成水溶性WP-Heme纳米复合物，其平均粒径为180～200 nm，Z-电位低于–20 mV；傅里叶变换红外光谱与耗散型石英晶体微天平分析发现WP和Heme之间发生结合，并且二者结合的粉末吸湿性明显降低，复溶性达到90%以上。通过构建WP-Heme纳米复合物解决了Heme在中性条件下的溶解问题，并将其进行喷雾干燥制成粉末，有望显著提高Heme的生物学价值并拓展其应用范围。更多还原

【Abstract】 In order to improve the water solubility of heme under neutral conditions,insoluble heme-peptide was first obtained by hydrolyzing porcine hemoglobin (PHb) with acid,and then whey protein-heme (WP-Heme) nanocomposites were constructed using whey protein and heme obtained from hydrolysis as raw materials,significantly improving the water solubility and bioavailability of heme.The results showed that under the condition of 85 ℃ and pH 2.0,PHb was hydrolyzed for 12 h,and the degree of hydrolysis reached more than 40%,obtaining the precipitation of heme-peptide hydrolytic mixture containing 16 amino acids,which contained 5% heme.Then,it interacted with whey protein under neutral conditions to produce water-soluble WP-Heme nanocomposites,with average particle sizes of 180–200 nm and Z-potential of less than–20 mV.Fourier transform infrared spectroscopy and quartz crystal microbalance with dissipation analysis showed that there was a combination between WP and heme,and the moisture absorption of the combined powder of the two was significantly reduced,and the resolubility reached more than 90%.The problem of heme dissolution under neutral conditions was solved by constructing WP-Heme nanocomposite,and it was made into powder by spray drying,which is expected to significantly improve the biological value of heme and expand its application range.更多还原