【摘要】 灰树花(Grifola frondosa)是我国近年来大力开发的药食用菌之一，灰树花多糖作为灰树花主要的生物活性成分，已经被证实具有抗肿瘤、免疫调节、抗辐射、降血糖和降血脂等作用，但其菌种普遍存在适应能力差、生长速度慢、多糖产量低等缺点。为获得生长速度块、多糖产量高的灰树花菌株，以灰树花CGMCC 5.404菌株为出发菌株进行筛选。首先利用紫外和常压室温等离子体(Atmospheric and room temperature plasma, ARTP)进行初筛，得到15株紫外诱变菌株和8株ARTP诱变菌株；继续利用基因组重排技术进行复筛，最终得到2株生长性能提高的基因组重排菌株G-31和G-59。G-31和G-59的生长速度分别为3.10 mm/d和2.79 mm/d，相较于原始菌株的生长速率提高了20.62%和8.56%；生物量分别为5.01 g/L和3.78 g/L，相较于原始菌株的2.62 g/L提高了91.22%和44.27%;G-31的单位菌体多糖产量为119.73 mg/g略低于原始菌株，G-59的单位菌体多糖产量为299.43 mg/g，相比原始菌株提高了67.75%。研究结果为工业化生产灰树花多糖提供了潜在的菌种资源，并为进一步的菌种改良和发酵工艺优化奠定了基础。更多还原

【Abstract】 Grifola frondosa, commonly known as the maitake mushroom, is one of the medicinal and edible fungi that has been vigorously developed in China in recent years. The polysaccharides from Grifola frondosa, the main bioactive components, have been proven to possess anti-tumor, immunomodulatory, anti-radiation, hypoglycemic, and hypolipidemic effects. However, the strains generally suffer from poor adaptability, slow growth rate, and low polysaccharide yield. In order to obtain strains of Grifola frondosa with faster growth and higher polysaccharide production, a screening process was initiated using the Grifola frondosa CGMCC 5.404 strain as the starting strain. Initially, ultraviolet(UV) and atmospheric and room temperature plasma(ARTP) were utilized for primary screening, resulting in fifteen UV-mutated strains and eight ARTP-mutated strains. Subsequently, genome shuffling technology was employed for secondary screening, ultimately yielding two strains, G-31 and G-59, with improved growth performance. The growth rates of G-31 and G-59 were 3.10 mm/d and 2.79 mm/d, respectively, which represents an increase of 20.62% and 8.56% compared to the growth rate of the original strain. The biomass of G-31 and G-59 was 5.01 g/L and 3.78 g/L, respectively, which is an increase of 91.22% and 44.27% compared to the original strain’s 2.62 g/L. The polysaccharide yield of G-31 was 119.73 mg/g, slightly lower than that of the original strain, while G-59’s polysaccharide yield was 299.43 mg/g, an increase of 67.75% compared to the original strain. The findings provide potential strain resources for the industrial production of Grifola frondosa polysaccharides and lay the foundation for further strain improvement and optimization of fermentation processes.更多还原