【摘要】 双歧杆菌在培养过程中存在不同生理状态的细胞亚群，影响菌体的代谢活性和活菌数的提高，因此本文以长双歧杆菌婴儿亚种B8762(Bifidobacterium longum subsp. infantis B8762,B8762)为研究对象，明确其在不同碳源下细胞亚群间的生长性能差异。本实验通过流式细胞分选技术，分别将乳糖、蔗糖和麦芽糖作为碳源进行高密度发酵后的B8762菌体进行亚群分选，比较分析不同细胞亚群的碳代谢和生长性能差异，以评估培养碳源对菌体细胞活性的影响。结果表明：不同碳源培养的B8762菌体活菌数和浊度存在显著差异（P<0.05），且存在不同代谢活性亚群。其中乳糖、蔗糖和麦芽糖培养的活细胞亚群果糖-6-磷酸磷酸酮解酶（Fructose-6-phosphate phosphoketolase enzyme,F6PPK）活性较损伤细胞亚群分别提高了1.47倍、2.42倍和2.83倍，且经重培养后蔗糖培养活细胞亚群活菌数达（2.46±0.01）×10~9 CFU/mL，显著高于其他亚群（P<0.05）。总的来说，相较于乳糖和麦芽糖，蔗糖作为碳源时菌体细胞活性最好，为活性优势细胞群体调控培养和制备高活性菌粉提供了新的思路和方法。更多还原

【Abstract】 Bifidobacterium exhibits distinct physiological subpopulations during cultivation, which influences its metabolic activity and enhancement of the number of viable bacteria. This study focused on Bifidobacterium longum subsp. infantis B8762(B8762) to explore the growth performance differences among its cell subpopulations under various carbon sources.Flow cytometry sorting was employed to separate B8762 cells following high-density fermentation with lactose, sucrose,and maltose as carbon sources. The study compared and analyzed the differences in carbon metabolism and growth performance across these subpopulations to assess the impact of different carbon sources on bacterial cell viability. The results revealed that the number and turbidity of B8762 cells varied depending on the carbon sources(P<0.05), with distinct subpopulations showing different metabolic activities. Specifically, the activity of fructose-6-phosphate phosphoketolase(F6PPK) in the live cell subpopulations cultured with lactose, sucrose, and maltose was 1.47, 2.42, and 2.83 times higher,respectively, compared to the damaged cell subpopulation. After re-cultivation, the viable bacteria count in the sucrosecultured live cell subpopulation reached(2.46±0.01)×10~9 CFU/mL, significantly surpassing that of the other subpopulations(P<0.05). In conclusion, sucrose outperforms lactose and maltose as a carbon source in promoting cell activity, offering new insights and methods for optimizing the cultivation and preparation of highly active bacterial powders for dominant cell populations.更多还原