【摘要】 为了使苯并噻二唑(benzothiazole, BTH)对葡萄品质的调控结果得以在葡萄酒中体现，该研究以‘蛇龙珠’葡萄为试材，观察BTH处理葡萄的细胞壁变化，优化浸渍工艺，探讨浸渍及BTH处理对葡萄醪酚类、颜色和香气组成的影响。结果表明，BTH处理膨大(PD)、转色(ZS)和成熟期(CS)葡萄果皮细胞壁厚度分别较对照(CK)增加了2.79倍、62.83%和1.60倍，膨大期处理对果皮细胞壁影响最大。BTH处理葡萄的优化浸渍工艺为添加36 mg/L果胶复合酶23℃浸渍46 h。优化浸渍工艺葡萄醪中BTH处理样的总酚、总类黄酮、香气浓度和色泽品质则均高于CK样。交互冗余分析(redundancy analysis, RDA)与偏最小二乘法判别分析(partial least squares discriminant analysis, PLS-DA)显示，BTH处理样香气浓度增加与颜色品质改善主要取决于总酚和总类黄酮的变化；优化浸渍工艺对直链脂肪族、萜烯和支链脂肪族香气化合物释放影响最大。综上，BTH处理能显著改善葡萄醪酚类物质的积累以及色泽和香气的品质，进而改善葡萄酒的品质。该研究结果可为BTH调控蛇龙珠葡萄酒品质的应用推广提供科学理论和实践参考。更多还原

【Abstract】 To make the regulation results of benzothiadiazole(BTH) on grape quality reflected in wine, for this study, ’Cabernet Gernischt’ grape was used as test material, and the cell wall changes of grape treated with BTH were observed.Optimized impregnation process, effects of impregnation, BTH treatment on phenolics, and color and aroma composition of grape mash were investigated.Results showed that compared with the control(CK), the cell wall thickness of grape skin in the BTH treatment of berry enlargement stage(PD), veraison(ZS), and ripening(CS) increased by 2.79 times, 62.83%, and 1.60 times, respectively, and the treatment at the enlargement stage had the greatest effect on the cell wall of the peel.The optimized impregnation process of BTH treated grapes was 36 mg/L pectin compound enzyme that impregnated at 23 ℃ for 46 h.The total phenols, flavonoids, aroma concentration, and color quality of BTH treated grape mash in the optimized impregnation process were higher than those of CK.Interactive RDA and PLS-DA analysis showed that the increase of aroma concentration and the improvement of color quality of BTH treated samples mainly depended on the variation total phenols and flavonoids.The optimized impregnation process had the greatest effect on the release of linear aliphatic, terpenes, and branched-chain aliphatic aroma compounds.In summary, BTH treatment can significantly improve the accumulation of phenolic substances in grape mash, the quality of color, and aroma, thereby improve the quality of wine.The research results can provide scientific theory and practical reference for the application and promotion of BTH in regulating the quality of Cabernet Gernischt wine.更多还原