【摘要】 目的 通过流行病学调查、质谱仪器方法联合,对一起由食用鹿肉引起的疑难食物中毒事件进行病因学研究。方法 采集疑似中毒食物和病例血液样本,先采用高分辨质谱非靶向方法进行毒因筛查与确证,再通过三重四极杆质谱靶向检测方法对样本中赛拉嗪含量进行定量分析。结果 疑似中毒食物(生鹿肉和炒鹿肉)以及3位病例的血清样本均检出赛拉嗪,在生鹿肉与炒鹿肉中含量分别为3.0μg/kg、395.9μg/kg,病例血清中赛拉嗪的含量分别为47.0、16.0、15.6 ng/mL。结论 本起事件确定为食用鹿肉导致的赛拉嗪中毒事件,先通过流行病学调查快速圈定中毒病因的鉴定范围,非靶向筛查方法为中毒病因快速鉴定确定提供了技术支持,为中毒事件的快速处置和临床精准救治提供了依据,同时对中毒样本进行靶向定量分析也为动物养殖用药标准的制定提供依据。更多还原

【Abstract】 Objective To analyze the cause of a complex food poisoning incident using a combination of non-targeted and targeted methods based on mass spectrometry. Methods Suspected contaminated food and blood samples from the cases were collected. After sample preparation, a high-resolution mass spectrometry non-targeted method was used for toxin screening, and a triple quadrupole mass spectrometry targeted detection method was applied for the quantitative analysis of xylazine in the samples. Results Xylazine was detected in both the suspected contaminated food(raw and fried venison) and the serum samples of 3 cases. The concentrations in raw and fried venison were 3.0 μg/kg and 395.9 μg/kg, while the concentrations in the cases’ serum were 47.0, 16.0 and 15.6 ng/mL, respectively. Conclusion This incident is confirmed as xylazine poisoning caused by venison ingestion. First, through epidemiological investigation, the scope for identifying the cause of poisoning can be quickly determined. The non-targeted method provides accurate evidence for clinical treatment, aiding in the diagnosis and management of such events. At the same time, the targeted quantitative analysis of samples also provides a basis for the formulation of drug use standards in farmed animals in the future.更多还原