【摘要】 目的 分析辽宁省大连市食源性疾病患者来源的副溶血性弧菌(Vibrio parahaemolyticus,VP)分子流行特点,毒力基因及耐药基因的分布特征。方法 收集了2023年大连市63株VP临床分离株,借助全基因组测序,多位点序列分型、毒力基因以及耐药基因分析,同时结合临床流行病学数据进化全面分析。结果 7—9月为VP的主要流行周期。序列分型(sequence type, ST)结果显示, ST3型为主要流行群。63株菌同时携带tlh和vcrD, tdh和vcrD2毒力基因的携带率为95.23%。所有菌株均携带blaCARB-47、CARB-20、tet (35)、tet (34)以及CRP耐药基因,其中1株ST3型菌株还携带氨基糖苷类、磺酰胺类等耐药基因。毒力基因携带类型和ST型存在一定关联,但耐药基因分布没有明显的聚类特征。全身症状患者的分离株均携带tdh和vcrD2。结论 ST3型是辽宁省大连市VP的主要流行群,广泛分布且携带毒力基因比例较高。并密切关注ST3型的耐药性传播风险。本研究为VP的防控提供了分子流行病学依据。更多还原

【Abstract】 Objective To analyze the molecular epidemiological characteristics, virulence gene profiles, and distribution of antibiotic resistance genes of Vibrio parahaemolyticus(VP) isolated from foodborne diseases patients in Dalian City, Liaoning Province. Methods A total of 63 VP clinical isolates were collected from Dalian City in 2023. The whole genome sequencing, multilocus sequence typing, virulence genes and drug resistance genes were analyzed, and the clinical epidemiological data were combined for comprehensive analysis. Results July-September was the main epidemic period of VP. The results of sequence type(ST) showed that ST3 was the main epidemic group. The 63 strains carried both tlh and vcrD, and the carrying rate of tdh and vcrD2 virulence genes was 95.24%.All strains carried blaCARB-47, CARB-20, tet(35), tet(34) and CRP resistance genes, and one ST3 strain also carried aminoglycosides, sulfonamides and other resistance genes. There was a certain correlation between virulence gene carrying type and ST type, but there was no obvious clustering feature in the distribution of drug resistance genes. All isolates from patients with systemic symptoms carried tdh and vcrD2. Conclusion ST3 is the main epidemic group of VP in Dalian, Liaoning Province, which is widely distributed and has a high proportion of virulence genes. Speical attention should be paid to the risk of drug resistance transmission of ST3. This study provides a molecular epidemiological basis for the prevention and control of VP.更多还原