【摘要】 食源性致病菌污染是全球食品安全领域的重大挑战,传统检测方法存在耗时长、操作复杂等局限性。近年来,基于噬菌体的生物传感器因其高特异性、灵敏度和快速检测能力,成为食源性致病菌检测的研究热点。噬菌体作为生物识别元件,能够特异性结合目标细菌,并通过光学、电化学、磁学等信号转换方式实现快速检测。本文综述了基于噬菌体的生物传感器在食源性致病菌检测中的研究进展,重点介绍了光学生物传感器、电化学生物传感器、磁生物传感器及多模式、多功能集成传感器的设计原理、技术优势及应用前景。这些传感器在食品安全、环境监测和临床诊断等领域展现出广阔的应用潜力。未来,随着噬菌体资源的丰富以及与生物信息学、人工智能技术的发展与融合,噬菌体生物传感器有望实现更高效的检测工具开发与规模化应用,为食品安全、甚至环境监测及临床诊断提供强有力的技术支持,具有重要研究意义与社会价值。更多还原

【Abstract】 Foodborne pathogens contamination is a major global challenge in the field of food safety. Traditional detection methods are limited by issues such as time-consuming processes and complex operational requirements. In recent years, bacteriophage-based biosensors have emerged as a promising research focus for the detection of foodborne pathogens due to their high specificity, sensitivity, and rapid detection capabilities. Bacteriophages, acting as biological recognition elements, can specifically bind to target bacteria and achieve rapid detection through signal transduction methods such as optical, electrochemical and magnetic signals. This review systematically summarized the research progress in bacteriophage-based biosensors for foodborne pathogenic bacteria detection, with a particular focus on the design principles, technical advantages and application prospects of optical biosensors, electrochemical biosensors, magnetic biosensors, and multimodal/multifunctional integrated sensors. These sensors show great potential for applications in food safety, environmental monitoring and clinical diagnostics. In the future, as bacteriophage resources become more abundant and with the advancement and integration of bioinformatics and artificial intelligence technologies, bacteriophage-based biosensors are expected to enable the development of more efficient detection tools and large-scale applications. These advancements will provide robust technical support for food safety, environmental monitoring and clinical diagnostics, holding significant research and societal value.更多还原