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合相色谱法定量检测蔬菜中的叶绿素含量

Quantitative determination of chlorophyll content in vegetables by convergence chromatography

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【作者】 陈山乔卢超艳尚海涛章豪吴银良范春波孙志栋

【Author】 CHEN Shan-Qiao;LU Chao-Yan;SHANG Hai-Tao;ZHANG Hao;WU Yin-Liang;FAN Chun-Bo;SUN Zhi-Dong;Ningbo Academy of Agricultural Sciences;Ningbo Key Laboratory of Testing and Control for Characteristic Agro-product Quality and Safety;Ningbo New Ziyuntang Aquatic Products Co., Ltd.;

【通讯作者】 孙志栋;

【机构】 宁波市农业科学研究院宁波市特色农产品质量安全检测与控制重点实验室宁波新紫云堂水产食品有限公司

【摘要】 目的 建立合相色谱法(convergence chromatography, CC)测定蔬菜中叶绿素a和叶绿素b的含量。方法 采用索氏抽提法对蔬菜及其制品中的叶绿素进行高效提取,并使用超高效合相色谱仪结合紫外-可见检测器进行分离与检测。结果 通过对不同溶剂稳定性的对比,选择了石油醚作为提取溶剂,并优化了提取条件。经验证,检出限为0.02 mg/kg,定量限为0.06 mg/kg,线性范围为0.06~800.00 mg/kg,加标回收率为99%~102%,真实样品相对标准偏差2.2%~14.0%。通过对真实样品,包括羽衣甘蓝、鲜芥菜、雪菜、梅干菜、蒸煮梅干菜、水煮梅干菜的检测精密度考察并与现行标准方法的交叉验证,证明了该方法的可靠性和准确性。结论 本研究建立了一种基于CC的检测蔬菜中叶绿素a和叶绿素b的方法,具有较高的灵敏度、较宽的线性范围和良好的特异性和精密度。该方法不仅适用于实验室研究,也具备实际应用潜力。

【Abstract】 Objective To establish a method for the determination of chlorophyll a and chlorophyll b content in vegetables by convergence chromatography(CC). Methods The Soxhlet extraction method was used for efficient extraction of chlorophyll from vegetables and their products, followed by separation and detection using an ultra-performance convergence chromatography system coupled with a ultraviolet-visible detector. Results Through comparison of the stability of different solvents, petroleum ether was selected as the extraction solvent, and the extraction conditions were optimized. The method was validated with a limit of detection of 0.02 mg/kg, a limit of quantification of 0.06 mg/kg, a linear range of 0.06–800.00 mg/kg, a spike recovery rate of 99%–102%, and a relative standard deviation of 2.2%–14.0% for real samples. The reliability and accuracy of the method were confirmed through precision testing of real samples, including kale, fresh mustard greens, pickled mustard greens, dried pickled mustard greens, steamed dried pickled mustard greens, and boiled dried pickled mustard greens, as well as cross-validation with existing standard methods. Conclusion This study establishes a CC-based method for detecting chlorophyll a and chlorophyll b in vegetables, demonstrating high sensitivity, a wide linear range, good specificity, and precision. The method is not only suitable for laboratory research but also has practical application potential.

【基金】 宁波市公益类项目(2022S140);绿色食品(绿色优质农产品)高质量创新发展技术研究项目(GFJY20240010);“科创甬江2035”关键技术突破计划项目(20241ZDYF020210);宁波市食品安全与营养健康重大专项(2022Z176)
  • 【文献出处】 食品安全质量检测学报 ,Journal of Food Safety & Quality , 编辑部邮箱 ,2025年06期
  • 【分类号】TS255.7;O657.7
  • 【下载频次】117
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