【摘要】 目的：观察电针百会、神庭穴对脑卒中后认知障碍（PSCI）模型大鼠的神经功能缺损情况、学习记忆功能和ABCG3l1的mRNA相对表达的影响，探讨其改善PSCI的作用机制。方法：将27只雄性SD大鼠按随机数字表法分为假手术组、模型组和电针组，每组9只。采用Zea Longa改良线栓法制备大脑中动脉栓塞（MCAO）模型，造模后第1天对电针组进行电针百会、神庭穴干预，20 min/次，1次/d，持续7 d。假手术组和模型组同等条件抓取，不进行电针干预。采用ZeaLonga 5分法对大鼠进行神经功能缺损评估，Morris水迷宫实验评估大鼠空间学习和记忆能力，实时定量荧光PCR(RTqPCR)检测海马组织中ABCG3l1的mRNA相对表达量，生物信息学方法分析ABCG3与ABCG3l1的同源性。结果：生物信息学分析得知ABCG3与ABCG3l1具有高度同源性；Zea-Longa 5分法中，造模后第1天、第7天，模型组和电针组神经功能评分均较假手术组升高，差异有统计学意义（P<0.01）；与模型组造模第7天和电针组第1天比较，电针组第7天神经功能评分均明显下降，差异有统计学意义（P<0.05）。在Morris水迷宫定位航行实验中，重复测量方差分析结果显示，组别主效应、干预时间主效应、组别与干预时间的交互效应均显著。Morris水迷宫空间探索实验检测结果表明，与假手术组比较，模型组大鼠的平台穿越次数明显减少，差异有统计学意义（P<0.05）；第4、5、6天测试数据显示，与假手术组比较，模型组大鼠平台探索总时长增加，差异有统计学意义（P<0.01）；与模型组比较，电针组大鼠平台探索总时长缩短，差异有统计学意义（P<0.01）。在RT-qPCR实验中，与假手术组比较，模型组ABG3l1 mRNA表达量明显增高，差异有统计学意义（P<0.05）；与模型组比较，电针组ABCG3l1 mRNA表达量明显降低，差异有统计学意义（P<0.05）。结论：电针百会、神庭可以改善PSCI模型大鼠的神经功能评分，提高大鼠空间学习和记忆能力，降低缺血侧海马组织中ABCG3l1的mRNA相对表达，改善PSCI大鼠的认知障碍，其机制与纠正海马组织中ABCG3l1的mRNA表达有关。更多还原

【Abstract】 Objective: To observe the effects of electroacupuncture at the Baihui and Shenting acupoints on neurological deficits, learning and memory functions and the relative mRNA expression of ABCG3l1 in model rats with post-stroke cognitive impairment(PSCI), and to explore its mechanism in improving PSCI. Methods: A total of 27 male SpragueDawley(SD) rats were randomly divided into a sham-operation group, a model group and an electroacupuncture group using a random number table method, with 9 rats in each group. The middle cerebral artery occlusion(MCAO) model was established using the modified Zea Longa suture-occluded method. On the first day after modeling, rats in the electroacupuncture group received electroacupuncture intervention at the Baihui and Shenting acupoints for 20 minutes per session, once a day, for 7 consecutive days. Rats in the sham-operation group and the model group were handled in the same way in terms of capture but did not receive electroacupuncture intervention. The Zea Longa 5-point scale was used to assess neurological deficits in rats. The Morris water maze test was employed to evaluate spatial learning and memory abilities of rats. Real-time quantitative PCR(RTqPCR) was used to detect the relative mRNA expression of ABCG3l1 in the hippocampus. Bioinformatics methods were applied to analyze the homology between ABCG3 and ABCG3l1. Results: Bioinformatics analysis revealed a high degree of homology between ABCG3 and ABCG3l1. According to the Zea Longa 5-point scale, on the 1st and 7th days after modeling, the neurological function scores of both the model group and the electroacupuncture group were higher than those of the sham-operation group, with statistically significant differences(P<0.01). Compared with the model group on the 7th day after modeling and the electroacupuncture group on the 1st day, the neurological function score of the electroacupuncture group on the 7th day was significantly decreased, with a statistically significant difference(P<0.05). In the place navigation test of the Morris water maze, repeated measures analysis of variance showed significant main effects of group, intervention time and their interaction. The results of the spatial probe test in the Morris water maze indicated that compared with the sham-operation group, the number of platform crossings in the model group was significantly reduced, with a statistically significant difference(P<0.05). Data from tests on the 4th, 5th and 6th days showed that compared with the sham-operation group, the total time spent exploring the platform in the model group was increased, with a statistically significant difference(P<0.01). Compared with the model group, the total time spent exploring the platform in the electroacupuncture group was shortened, with a statistically significant difference(P<0.01). In the RT-qPCR experiment, compared with the sham-operation group, the mRNA expression level of ABCG3l1 in the model group was significantly increased, with a statistically significant difference(P<0.05). Compared with the model group, the mRNA expression level of ABCG3l1 in the electroacupuncture group was significantly decreased, with a statistically significant difference(P<0.05). Conclusion: Electroacupuncture at the Baihui and Shenting acupoints can improve the neurological function scores, enhance spatial learning and memory abilities, reduce the relative mRNA expression of ABCG3l1 in the ischemic hippocampus, and improve cognitive impairment in PSCI model rats. The mechanism may be related to correcting the mRNA expression of ABCG3l1 in the hippocampus.更多还原