【摘要】 目的:探讨多囊卵巢综合征(PCOS)稽留流产患者蜕膜组织中整合素αvβ3及骨桥蛋白(OPN)的表达。方法:选择2018年2月至2022年12月就诊于西安交通大学第一附属医院的PCOS稽留流产(PCOS稽留流产组)、非PCOS稽留流产(非PCOS稽留流产组)和正常早期妊娠人工流产(正常对照组)女性各20例为研究对象，检测3组蜕膜组织中整合素αvβ3、OPN的表达。分析PCOS稽留流产患者蜕膜组织中整合素αvβ3、OPN表达的相关性。结果:整合素αvβ3、OPN在蜕膜组织中主要表达于子宫腺上皮细胞，同时也表达于蜕膜细胞。3组蜕膜组织中整合素αvβ3、OPN的阳性率和免疫组化(IHC)染色评分比较差异有统计学意义(P<0.05),且PCOS稽留流产组显著低于非PCOS稽留流产组和正常对照组(P<0.05),而非PCOS稽留流产组与正常对照组比较差异无统计学意义(P>0.05)。蜕膜组织中整合素αvβ3、OPN主要共定位于子宫腺上皮细胞，3组整合素αvβ3、OPN免疫荧光强度比较差异有统计学意义(P<0.05),且PCOS稽留流产组显著低于非PCOS稽留流产组和正常对照组(P<0.01),而非PCOS稽留流产组与正常对照组比较差异无统计学意义(P>0.05)。3组蜕膜组织中整合素αvβ3、OPN的蛋白及和mRNA的表达水平差异有统计学意义(P<0.05),且PCOS稽留流产组显著低于非PCOS稽留流产组和正常对照组(P<0.01),而非PCOS稽留流产组与正常对照组差异无统计学意义(P>0.05)。Pearson相关分析结果显示，PCOS稽留流产患者蜕膜组织中整合素αvβ3的蛋白及mRNA的表达水平与OPN均呈显著正相关(r>0,P<0.01)。结论:整合素αvβ3、OPN在早期妊娠的子宫腺上皮细胞和蜕膜细胞中均有表达，两者在PCOS稽留流产蜕膜组织中的表达显著降低，可能与子宫内膜容受性降低有关，其在PCOS患者稽留流产中的作用仍需探索。更多还原

【Abstract】 Objective:to investigate the expression of integrin αvβ3 and osteopontin(OPN) in decidual tissues in missed abortion women with polycystic ovarian syndrome(PCOS).Methods:PCOS women with missed abortion(PCOS missed abortion group),non-PCOS with missed abortion(non-PCOS missed abortion group) and women with normal early pregnancy induced abortion(normal control group) who were admitted to the First Affiliated Hospital of Xi ′an Jiaotong University from February 2018 to December 2022 were selected as participants, and 20 subjects in each group.The expressions of integrin αvβ3 and OPN in decidual tissues among the three groups were detected.Correlation analysis was conducted between the expression levels of integrin αvβ3 and OPN in PCOS patients with missed abortion.Results:Integrin αvβ3 and OPN were mainly expressed in the epithelial cells of the uterus, as well as in decidual cells.There were significant differences in the positive rates and IHC scores of integrin αvβ3 and OPN in decidual tissues among the three groups(P<0.05).The positive rates and IHC scores in PCOS missed abortion group were significantly lower compared with non-PCOS missed abortion group and normal control group, and the difference was statistically significant(P<0.05),but there was no significant difference between non-PCOS missed abortion group and normal control group(P>0.05).The integrin αvβ3 and OPN in decidual tissues were mainly co-located in uterine epithelial cells.There were significant differences in immunofluorescence intensity of integrin αvβ3 and OPN among the three groups(P<0.05).The missed abortion group of PCOS was significantly lower than that of non-PCOS missed abortion group and normal control group, and the difference was statistically significant(P<0.01).No significant difference was found between non-PCOS missed abortion group and normal control group(P>0.05).There were significant differences in the protein expression levels and mRNA expression levels of integrin αvβ3 and OPN in decidual tissue among the three groups(P<0.05).PCOS missed abortion group was significantly lower than non-PCOS missed abortion group and normal control group, and the difference was statistically significant(P<0.01).There was no significant difference between non-PCOS missed abortion group and normal control group(P>0.05).The results of Pearson correlation analysis showed that the protein and mRNA expression levels of integrin αvβ3 and OPN in decidual tissue of PCOS patients with missed abortion were significantly positively correlated(r>0,P<0.01).Conclusions:The integrin αvβ3 and OPN were expressed in both epithelial cells of the uterus and decidual cells in early pregnancy.The expressions of them were significantly decreased in decidual tissues of PCOS patients with missed abortion, which might be related to reduced endometrial receptivity.The role of integrin αvβ3 and OPN in the occurrence of missed abortion needs further study.更多还原