【摘要】 目的 本研究以白术多糖为研究对象，探究其最佳提取方法，并对其进行初步结构表征、抗氧化及免疫活性的探究。方法 白术中的多糖含量通过苯酚硫酸法进行测定，并通过多糖含量作为考察指标以探究白术中多糖成分提取的最佳方法；结构主要通过红外光谱(IR)、高效凝胶渗透色谱法(HPGPC)、高效液相色谱法(HPLC)等手段对其官能团、分子量及单糖组成进行表征；通过对DPPH·及·OH自由基的检测来探究白术多糖的抗氧化活性；以RAW264.7细胞为研究对象，采用MTT法及流式细胞术探究白术多糖的免疫活性。结果 白术通过热水浸提法所得的多糖含量最高；通过红外光谱发现白术多糖含有O-H键、C-H键、C-O键等，且白术多糖的糖苷键可能具有α和β两种构型；HPGPC结果发现白术多糖是一个单一对称峰，表明是均一多糖，且分子量为3 011.5 Da; 1-苯基-3-甲基-5-吡唑啉酮(PMP)-HPLC结果表明白术多糖的单糖组成主要包括Man、Rha、GalA、Glc、Xyl、Ara,其摩尔比为1.00∶1.38∶4.88∶51.88∶6.88∶8.00。白术多糖具有一定的DPPH·和·OH清除效果，发挥显著的抗氧化活性；且白术多糖能显著促进RAW264.7细胞的增殖及吞噬活性，具有显著的免疫活性。结论 通过热水浸提法可以得到均一的白术多糖，并表现出显著的抗氧化及免疫活性。更多还原

【Abstract】 Objective The aim of this study was to investigate the optimised extraction method, structural characterization, antioxidant activity and immunological activity of polysaccharides from Atractylodes macrocephala Koidz..Methods The polysaccharide content was determined by phenol sulfuric acid method, and the optimal extraction method of Atractylodes macrocephala Koidz.was investigated using the polysaccharide content as an index; The functional groups, molecular weights and monosaccharide compositions were determined by infrared spectrometry, HPGPC,and PMP-HPLC,respectively; The antioxidant activities of were probed by DPPH· and ·OH radicals; RAW264.7 cells were used to investigate the immunological activity by MTT method and flow cytometry.Results The optimal extraction method for Atractylodes macrocephala polysaccharide was hot water extraction; Infrared spectroscopy results showed that Atractylodes macrocephala polysaccharide contained O-H,C-H and C-O bond, etc.,and had α and β configuration; HPGPC results showed that Atractylodes macrocephala polysaccharide was a homogeneous polysaccharide with a molecular weight of 3 011.5 Da; PMP-HPLC results showed that Atractylodes macrocephala polysaccharide was composed of Man, Rha, GalA,Glc, Xyl, and Ara with a molar ratio of 1.00∶1.38∶4.88∶51.88∶6.88∶8.00;Atractylodis macrocephalae polysaccharide showed a certain antioxidant activity with better DPPH· and·OH-scavenging effects; Furthermore, they significantly promoted the proliferation and phagocytosis activity of RAW264.7 cells, and possessed significant immunological activity.Conclusion A homogeneous polysaccharide of Atractylodes macrocephala could be obtained by hot water extraction and showed significant antioxidant and immunological activities.更多还原