【摘要】 针对离体监测药代动力学数据的采集过程易受样本量不足、数据采集延迟和个体间差异显著影响的问题，本研究通过集成硬件组件和LabView平台，开发了一套能够实时采集数据的四通道在体荧光监测系统。该系统基于激光诱导荧光原理，利用光电倍增管(PMT)特有的增益机制放大微弱的光信号。通过对背景光和0.25μg/mL异硫氰酸荧光素(FITC)溶液中的荧光光子数进行单因素方差分析(P<0.05),验证该系统的灵敏度和有效性。在体实验进一步证明，该系统不仅能有效实现对肾脏等主要代谢器官荧光信号的实时监测，还能最大限度地减少离体实验引起的数据误差，提高数据准确性，为后续药物疗效和治疗机制的研究提供更全面的数据。更多还原

【Abstract】 Aiming at the problems that the collection process of pharmacokinetic data for ex vivo monitoring is easily affected by insufficient sample size, delayed data collection and significant differences among individuals, we developed a four-channel in vivo fluorescence monitoring system with real-time data acquisition by integrating hardware components and the LabView platform. Based on the principle of laser-induced fluorescence, this system utilized the unique gain mechanism of photomultiplier tube(PMT) to amplify weak optical signals. The sensitivity and effectiveness of the system were confirmed by one-way analysis of variance(P<0.05) on the background light and the number of fluorescent photons in 0.25 μg/mL fluorescein isothiocyanate(FITC) solution. In vivo experiment further demonstrated that this system could not only effectively achieve real-time monitoring of fluorescence signals in major metabolic organs such as the kidneys, but also minimized data errors caused by ex vivo experiment and improve data accuracy. It can provide more comprehensive data for subsequent research on drug efficacy and therapeutic mechanisms.更多还原