【摘要】 肿瘤具有高度复杂且独特的生理结构,化疗和放疗等传统治疗手段难以彻底清除癌细胞.细菌因独特的生物学特性能够有效浸润肿瘤组织并发挥良好的抑瘤效应.然而天然细菌存在高毒副作用的缺陷,无法满足治疗中对安全性和有效性的要求.近年来,随着合成生物学与材料科学的快速发展,为开发出兼具低毒特性和多重治疗功能的工程化细菌提供了技术支持.本综述梳理了近些年工程化细菌研究领域的最新进展,通过阐明工程化细菌疗法的独特优势与作用机制,深入探讨了基于合成生物学技术修饰的工程化细菌构建策略,并对此种疗法现存挑战及未来发展方向进行了讨论.更多还原

【Abstract】 The unique physiological characteristics and complexity of tumor, in addition to drug resistance result in traditional therapies, such as chemotherapy and radiotherapy, being unable to achieve complete elimination of cancer cells. Meanwhile, the emerging immunotherapy suffers from a low patient response rate. Bacterial therapies are highly targeted. Bacteria can penetrate deep into the tumor and show good tumor inhibition. However, natural bacteria have the limitation of high toxicity and inability to meet the demand for efficient therapeutics. Recent advances in synthetic biology and materials science relate to the safety and efficacy of bacterial therapeutics, promising to develop engineered bacteria with low toxicity and complex therapeutic functions. Engineered bacteria that express anticancer drug molecules can target the tumor region, synthesizing and releasing payloads in response to internal and external stimuli. This process leads to the regression of the tumor and the effective inhibition of recurrence. This review outlines the recent advancements in the field of engineered bacteria research, particularly focusing on their applications in anti-tumor therapy. It also includes the advantageous features and mechanisms of engineered bacteria therapy, synthetic biology modification methods, and future challenges and directions of engineered bacteria therapy.更多还原