【摘要】 揭示不同补藻模式下的菌藻协同机制，为优化菌藻颗粒污泥工艺提供理论依据。采用2组序批式反应器（SBR），以连续补藻（A）和间歇补藻（B）模式培养菌藻颗粒污泥，进水为合成废水，小球藻为藻种，探究SBR运行过程中补藻策略对颗粒特性、除污效能、微生物群落组成的影响，以及菌藻颗粒形成的机制。结果表明，A和B的菌藻颗粒污泥成熟后，平均粒径分别为（0.78±0.08）和（0.97±0.05）mm，叶绿素a含量分别为（4.83±0.04）和（5.76±0.23）mg/g；对COD、NH₄⁺-N和TP的平均去除率分别为94.39%、87.99%、88.86%和94.45%、88.19%、91.12%,B更有利于废水中污染物去除。A、B处理的优势菌属为动胶菌属、陶厄氏菌属、黄杆菌属和聚磷菌，陶厄氏菌属是EPS中多糖（PS）增加的来源。A颗粒污泥的形成取决于藻类黏附生长，B则为藻类屏障作用。与A相比，B促进了胞外聚合物（82.26±3.86）mg/g的分泌，维持了颗粒结构。采用间歇补藻策略培养菌藻颗粒有效缩短了颗粒的形成时间（提前10 d），增强细菌聚集和污染物降解性能（除碳）。更多还原

【Abstract】 In this study, we explored the effects of different algae replenishment strategies on sludge granule characteristics, pollutant removal efficiency, and microbial community composition, as well as the mechanisms of algal-bacterial granule formation in the sequencing batch reactor（SBR） system. The aim of the study was to reveal the mechanisms of algal-bacterial synergy under different augmentation strategies and provide a theoretical foundation for optimizing the algal-bacterial granular sludge process.Two algae replenishment strategies（treatment A: continuous algal feeding; treatment B: intermittent algal feeding） were set to culture bacteria-algae granular sludge, with synthetic wastewater as the input and chlorella as the algae species in the SBR system. When the algal-bacterial granules had matured, the mean granule sizes were（0.78±0.08） mm in treatment A and（0.97±0.05） mm in treatment B. The contents of chlorophyll-a were（4.83±0.04） mg/g in treatment A and（6.28±0.04） mg/g in treatment B, and the average removal rates of COD, NH₄⁺-N and TP from the wastewater were, respectively, 94.39%, 87.99%, and 88.86% in treatment A and 94.45%, 88.19%, and 91.12% in treatment B. Intermittent algae augmentation enhanced the pollutant removal capacity of algal-bacterial granular sludge, and facilitated the colonization of algae on the granule surface, increased algal cell growth, and stimulated the release of extracellular polymeric substances（EPS） by both algae and bacteria. The dominant bacteria genera in treatments A and B were Zoogloea, Thauera, Flavobacterium and Candidatus Accumulibacter（phosphorus accumulating bacteria）. Thauera was the source of increased polysaccharide（PS） in the EPS. In addition, the formation of granular sludge in treatment A primarily depended on algal adhesion and growth, while treatment B utilized an algal barrier. The intermittent algae replenishment strategy for algae-bacteria granule cultivation significantly shortened the formation of granules（10 days） and enhanced bacterial aggregation and degradation of carbonaceous pollutants.更多还原