【摘要】 为改善香草兰挥发油水溶性差、稳定性低等问题，以马达加斯加香草兰豆荚提取得到的香草兰挥发油作为主要原料，采取自发乳化法构建香草兰挥发油纳米微乳化体系并对其稳定性进行评价。结果表明：香草兰挥发油纳米微乳液最佳组成为表面活性剂Tween 40与助表面活性剂丙二醇的质量比为2，混合表面活性剂与油相的质量比为9∶1，可得到水包油型（O/W）香草兰挥发油纳米微乳液，纳米微乳液的粒径为(16.75±0.06)nm，聚合物分散系数（PDI）为0.240±0.003,Zeta电位为(–3.15±0.46)V。采用离心法、加热法以及低温贮藏法对香草兰挥发油纳米微乳液进行稳定性评价。结果表明，在离心速率为2000～10 000 r/min，加热温度为40～90℃条件下，香草兰挥发油纳米微乳液能保持澄清、透明状态，在4℃条件下放置30d，未产生浑浊、沉淀等现象，表明香草兰挥发油纳米微乳液具有良好的离心稳定性、热稳定性以及贮藏稳定性。研究结果为香草兰挥发油的应用提供一定的技术支撑。更多还原

【Abstract】 In order to solve the problems of poor water solubility and low stability of vanilla volatile oil, the vanilla volatile oil extracted from Madagascar vanilla pods was used as the main raw material, and the nano-emulsification system of vanilla volatile oil was constructed by the spontaneous emulsification method and stability was evaluated. The results showed that the optimal process formula of vanilla volatile oil nano-microemulsion was the mass ratio of surfactant to co-surfactant was 2, and the mass ratio of mixed surfactant to oil phase was 9∶1, and O/W vanilla volatile oil nano-microemulsion could be obtained, and the particle size of the nano-microemulsion was(16.75±0.06)nm, and the polymer dispersity index(PDI) was 0.240±0.003. The Zeta potential was(–3.15±0.46)V. The results showed that the vanilla volatile oil nano-microemulsion could remain clear and transparent under the conditions of centrifugation rate of 2000-10 000 r/min and heating temperature of 40–90 ℃, and was placed at 4 ℃ for 30 days without turbidity and precipitation, indicating that the vanilla volatile oil nano-emulsion had good centrifugal stability, thermal stability and storage stability. The results would provide some technical support for the application of vanilla volatile oil and nano microemulsion.更多还原