【摘要】 目的 分析成都市新都区2014—2020年8起副溶血性弧菌（Vibrio parahaemolyticus, VP）食源性暴发事件分离株的分子分型特征和耐药特征，为新都区防控副溶血性弧菌食源性暴发提供科学依据。方法 采用血清学分型、毒力基因检测、药敏试验、脉冲场凝胶电泳（pulsed field gel ectrophoresis, PFGE）实验对92株副溶血性弧菌进行分析。结果 92株菌共检出O1、O2、O3、O4、O8 5个血清群，O1群（52株，56.52%）和O4群（30株，32.61%）为优势血清群；人员分离株tdh基因携带率97.37%(74/76)，其中有1株2014年患者分离株O4:K9同时携带了tdh和trh基因；食品和环境分离株均不携带tdh和trh基因；对头孢唑啉、多黏菌素E的耐药率分别为93.48%(86/92)、88.33%(53/60)，其中多重耐药率为1.09%(1/92)，耐药谱CT-CFZ-SXT。8起事件92株菌PFGE分子分型可分为36种带型，同一起事件的菌株带型多样，其中有2起事件的患者分离株和食品、环境分离株具有相同带型，也有4起不同事件具有2条相同带型。结论 成都市新都区2014—2020年副溶血性弧菌食源性暴发事件多为2种血清型以上的混合感染，且PFGE型别多样；菌株对头孢唑啉和多粘菌素E耐药率高。建议副溶血性弧菌导致暴发事件时，可从同一样本中多分离菌株进行分析，也可开展高通量测序进行溯源分析，为防控副溶血性弧菌食源性暴发提供数据支撑。更多还原

【Abstract】 Objective To analyze molecular typing and drug resistance characteristics of isolates from 8 foodborne outbreaks of Vibrio parahaemolyticus(VP)in Xindu District, Chengdu from 2014 to 2020, in order to provide scientific basis for control and prevention of foodborne outbreaks of Vibrio parahaemolyticus in Xindu District.Methods Totally 92 strains of Vibrio parahaemolyticus were analyzed by serotype, virulence gene test, drug sensitivity test and pulsed field gel electrophoresis.Results All 92 strains of Vibrio parahaemolyticus were detected with five serum groups: O1, O2, O3, O4, and O8. O1 group(52 strains, 56.52%) and O4 group(30 strains, 32.61%) were the dominant serum groups; tdh gene carrying rate of human isolates was 97.37%(74/76), among which one patient isolate O4:K9 from 2014 carried both tdh and trh genes; Both food and environmental isolates didn’t carry tdh and trh genes; The resistance rates to cefazolin and polymyxin E were 93.48%(86/92) and 88.33%(53/60), respectively, with a multidrug resistance rate of 1.09%(1/92). The resistance spectrum was CTCFZ-SXT. The PFGE molecular typing of 92 strains in 8 events could be divided into 36 band types. The strains from the same incident presented diverse banding patterns. Among them, the isolates from patients, food and environment in 2 incidents shared the same banding pattern, and 2 identical banding patterns were also found in 4 different incidents.Conclusion The foodborne outbreaks of Vibrio parahaemolyticus in Xindu District, Chengdu from 2014 to 2020 were mostly mixed infections of two or more serotypes, and the PFGE types were diverse; The strain has a high resistance rate to cefazolin and polymyxin E. It is suggested that when Vibrio parahaemolyticus causes an outbreak, multiple strains can be isolated from the same sample for analysis, or high-throughput sequencing can be conducted for traceability analysis to provide data support for control and prevention of foodborne outbreaks of Vibrio parahaemolyticus.更多还原