【摘要】 目的 探究淡色库蚊对残杀威抗性的表皮蛋白适应机制，明确差异表达蛋白在抗性与敏感株系抗性形成中的作用。方法 以实验室淡色库蚊敏感株系为亲本，连续20代残杀威筛选培育抗性株系，收集抗性与敏感株淡色库蚊各发育阶段样本。采用同位素标记相对和绝对定量技术（isobaric tags for relative and absolute quantification, iTRAQ）定量分析蛋白组，利用COG、GO和KEGG数据库进行功能注释及通路富集分析，并通过平行反应监测验证关键蛋白表达。结果 共鉴定出156个差异表达蛋白（59个上调，97个下调），淡色库蚊抗性株系中表皮结构成分蛋白（B0WIY4、B0W0L1）、几丁质结合蛋白及幼虫表皮蛋白显著上调；细胞骨架相关蛋白（B0WY76）、能量代谢蛋白（丙酮酸脱氢酶B0XA87）及核糖体蛋白（B0WQU6）显著下调。平行反应监测验证了肌动蛋白（B0WY76）等11类蛋白表达。结论 淡色库蚊对残杀威抗性与表皮蛋白差异表达和细胞骨架重构引起的表皮结构改变密切相关，这种结构改变可能延缓杀虫剂表皮渗透，从而增强对残杀威的抗性。更多还原

【Abstract】 Objective To investigate the potential cuticular protein adaptation mechanisms of Culex pipiens pallens resistance to propoxur for characterizing the functional roles of differentially expressed proteins(DEPs) in conferring the resistance between resistant and susceptible strains. Methods A propoxur-resistant strain was generated through 20generations of selective pressure from a laboratory-maintained susceptible strain. Samples spanning multiple developmental stages from both strains were subjected to quantitative proteomic profiling using isobaric tags for relative and absolute quantification(iTRAQ). Subsequent functional annotation and pathway enrichment analyses were performed by means of COG, GO, and KEGG databases, followed by targeted validation of pivotal proteins via parallel reaction monitoring(PRM). Results A total of 156 DEPs, comprising 59 upregulated and 97 downregulated proteins, were identified. Cuticular structural components(B0WIY4, B0W0L1), chitin-binding proteins, and larval cuticular proteins exhibited marked upregulation in the resistant strain, whereas cytoskeleton-associated proteins(B0WY76), energy metabolism regulators(pyruvate dehydrogenase B0XA87), and ribosomal proteins(B0WQU6) were significantly downregulated.PRM analysis confirmed the expression trends of 11 representative proteins, including actin(B0WY76). Conclusion The resistance of Culex pipiens pallens to propoxur is closely related to the structural alterations of the cuticle caused by differential expression of cuticular proteins and cytoskeletal remodeling. These alterations likely impede insecticide penetration through the cuticle, thereby enhancing propoxur resistance.更多还原