【摘要】 利用UPLC-Triple-TOF/MS技术鉴定沙棘提取物(Sea buckthorn extract,SBE)中的活性成分,并基于网络药理学方法预测可能发挥的抗炎活性成分及其作用靶点,进行分子对接验证,同时借助脂多糖(LPS)诱导RAW 264.7细胞模型进行抗炎活性分析。结果表明,从SBE中共鉴定出了13种化学成分,包括2个糖苷类、1个糖类、1个有机酸类、1个羧酸类、1个糖醛酸类、1个酚酸类以及6个黄酮类化合物。网络药理学筛选出沙棘提取物与炎症靶点共有164个靶点,推测其作用机制可能是作用于IL6、TNF-α和STAT3等核心靶点。分子对接结果显示,沙棘提取物中的化学成分与关键靶点具有较好的结合活性。体外细胞实验结果表明,沙棘提取物可显著降低LPS诱导的一氧化氮(NO)释放及促炎因子肿瘤坏死因子-α、白细胞介素-6 (IL-6)的表达水平(P<0.05或P<0.01)。因此得出沙棘提取物具有抗炎活性,其作用是通过多成分、多靶点、多通路机制发挥抗炎作用。该研究结果可为沙棘提取物的后续研究开发提供理论支撑。更多还原

【Abstract】 The active constituents of sea buckthorn extract(SBE) were identified using UPLC-Triple-TOF/MS technology.A network pharmacology approach was employed to predict the potential anti-inflammatory components and their corresponding targets,followed by molecular docking validation.The anti-inflammatory activity was further evaluated using a lipopolysaccharide(LPS)-induced RAW 264.7 cell model.A total of 13 chemical components were identified in SBE,including 2 glycosides,1 sugar,1 organic acid,1 carboxylic acid,1 uronic acid,1 phenolic acid,and 6 flavonoids.Network pharmacology analysis revealed 164 common targets between SBE and inflammationrelated pathways,suggesting that its mechanism may involve core targets such as IL6,TNF-α,and STAT3.Molecular docking results indicated favorable binding activity between the chemical components of SBE and these key targets.In vitro cell experiments demonstrated that SBE significantly reduced the release of nitric oxide(NO) and the expression levels of the pro-inflammatory cytokines tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) induced by LPS(P <0.05 or P <0.01).In conclusion,Sea buckthorn extract exhibits anti-inflammatory activity,which is mediated through a multi-component,multi-target,and multi-pathway mechanism.This study provides a theoretical foundation for subsequent research and development.更多还原