【摘要】 为探究低温胁迫条件下内生真菌发酵液浸种对燕麦生长及真菌群落结构的影响，进而筛选能够显著促进燕麦生长的内生菌株，以分离自青藏高原不同植物的内生青霉属真菌(Penicillium goetzii 204、Penicillium crustosum 132、Penicillium sp. 303)和木霉属真菌(Trichoderma sp.DK、Trichoderma alni 227)为研究对象，在低温胁迫条件下用菌种发酵液浸种燕麦(Avena sativa L.)种子，探究其对燕麦种子萌发、植株生长和生理特性的影响，并采用Illumina Miseq测序技术分析内生真菌的多样性。结果表明：真菌发酵液浸种会显著提高燕麦的根长(>6.08%)、根粗(>2.94%)、茎粗(>4.86%)、脯氨酸含量(>16.68%)及可溶性糖含量(>2.63%)。其中，木霉属菌株对燕麦生长和生理指标的促进作用显著优于青霉属菌株。在低温胁迫下，燕麦各组织Illumina Miseq测序共获得102 038条有效序列，包括4 412个OTU,隶属于9门20纲42目86科130属。真菌发酵液浸种后，燕麦根、茎、叶中的核心OTU数为113,浸种组各组织的特有OTU数量明显高于对照组。根与茎中出现的特有门为Zoopagomycota、Chytridiomycota和Rozellomycota,主要优势科为Olpidiaceae、Pleosporaceae和Russulaceae,优势属为Olpidium、Alternaria和Lactarius,Olpidium在茎与叶中的相对丰度显著增加。综上，内生真菌发酵液浸种可显著增加燕麦真菌群落的多样性，改善根际微生态环境，从而促进燕麦生长。更多还原

【Abstract】 This study aimed to investigate the effects of seed soaking with endophytic fungi fermentation broth on oat growth and fungal community structure under low-temperature stress and screen endophytic strains with significant growth-promoting effects. Endophytic Penicillium species(P.goetzii 204,P.crustosum 132,and Penicillium sp.303) and Trichoderma species(Trichoderma sp.DK and T.alni 227)isolated from different plants on the Qinghai-Xizang Plateau were used for study.Oat(Avena sativa L.)seeds were soaked with fungi fermentation broth under low-temperature stress, and the effects on seed germination, plant growth, and physiological characteristics were analyzed.Illumina MiSeq sequencing was employed for analyzing the diversity of endophytic fungi.The results showed that seed soaking with fungi fermentation broth significantly increased oat root length(>6.08%),root diameter(>2.94%),stem diameter(>4.86%),proline content(>16.68%),and soluble sugar content(>2.63%).Trichoderma strains significantly promoted oat growth and physiological indicators, outperforming Penicillium strains.Under low-temperature stress, Illumina MiSeq sequencing of oat tissues yielded 102 038 valid sequences and 4 412 OTUs, which belonged to 9 phyla, 20 classes, 42 orders, 86 families, and 130 genera.After seed soaking with fungi fermentation broth, the number of core OTUs in oat roots, stems, and leaves was 113,and the number of unique OTUs in each tissue of the seed soaking group was significantly higher than that of the control group.Unique phyla in roots and stems included Zoopagomycota, Chytridiomycota, and Rozellomycota.The dominant families were Olpidiaceae, Pleosporaceae, and Russulaceae, and the dominant genera were Olpidium,Alternaria,and Lactarius.The relative abundance of Olpidium increased significantly in stems and leaves.In summary, seed soaking with endophytic fungi fermentation broth significantly increased fungal community diversity, improved the rhizosphere microenvironment, and thereby promoted growth of oats.更多还原