【摘要】 正丙苯(n-propylbenzene, n-PBZ)和异丙苯(isopropylbenzene, i-PBZ)属于有毒有害物质，一旦泄漏入海将对海洋生态造成严重危害。前期研究发现，网状鼻胞藻S6A(Rhinomonas reticulata S6A)在7 d内对浓度5 mg/L的n-PBZ和i-PBZ的去除率分别为34.6%和38.8%。为进一步明确该微藻用于n-PBZ和i-PBZ污染海域修复所需的适宜环境条件，本研究采用基于Box-Behnken实验设计的响应面法，考察盐度、温度和光强对网状鼻胞藻S6A去除海水中n-PBZ和i-PBZ的影响。研究表明，影响n-PBZ和i-PBZ去除的关键因素分别为光强和温度，且温度分别与盐度、光强存在显著交互作用。微藻去除n-PBZ的最佳环境条件为盐度27.2、温度25.1℃和光强237.9μmol/(m~2·s),在此条件下实测的去除率达到51.2%;去除i-PBZ的最佳环境条件：盐度28.4、温度25.5℃和光强580.8μmol/(m~2·s),在此条件下实测的去除率达到54.6%。根据实验结果构建的去除率预测模型可用于预估n-PBZ和i-PBZ污染海域的生物修复效果，为确定使用微藻的适宜场合和时机提供决策依据。更多还原

【Abstract】 N-propylbenzene(n-PBZ) and isopropylbenzene(i-PBZ) are considered hazardous and noxious substances, and once leaked into the sea, they will cause serious harm to the marine ecosystems. Our previous study has found that the removal rate of n-PBZ and i-PBZ at a concentration of 5 mg/L by Rhodomonas reticulata S6A within 7 days were 34.6% and 38.8%, respectively. To further clarify the suitable environmental conditions for the remediation of propylbenzene-contaminated seawaters, the effects of salinity, temperature and light intensity on the removal of n-PBZ and i-PBZ from seawater by R. reticulata S6A were investigated in this study using response surface methodology(RSM) based on the Box-Behnken design. The results indicated that light intensity and temperature were the key factors affecting the removal of n-PBZ and i-PBZ, respectively. Additionally, the interactions between temperature and salinity, as well as temperature and light intensity, were found to be significant. The optimal environmental condition for the microalga to remove n-PBZ was as follows, salinity 27.2, temperature 25.1 ℃ and light intensity 237.9 μmol/(m~2·s) with a measured removal rate of 51.2%. The most suitable environmental condition for removing i-PBZ was as follows, salinity 28.4, temperature 25.5 ℃ and light intensity 580.8 μmol/(m~2·s) with a measured removal rate of 54.6%. The removal rate prediction model constructed based on the experimental results could be used to estimate the bioremediation effect in propylbenzene-contaminated seawaters, providing a decision-making basis for determining the appropriate occasions and times for using the microalga.更多还原