【摘要】 为探究电解式臭氧水对葡萄果实表面微生物菌落的影响，本研究采用16S rDNA和ITS2区域高通测序技术，分析了喷施不同浓度电解式臭氧水、苯醚甲环唑、清水的转熟期‘新雅’葡萄果面细菌和真菌群落。结果显示，喷施电解式臭氧水处理组葡萄果面的细菌以变形菌门、放线菌门、厚壁菌门、拟杆菌门为主；真菌以子囊菌门和担子菌门为主，优势属为曲霉菌属、枝孢菌属、螨霉菌属、外担菌属与青霉菌属。尽管菌落多样性指数无显著改变，但5 mg·L^-1电解臭氧水处理即能有效降低葡萄表面假单胞菌属、青霉菌属等有害病原菌的相对丰富度，且优于苯醚甲环唑处理。结果表明，喷施电解式臭氧水可调控葡萄果面微生物群落结构，抑制一些病原菌的繁殖，为其生产应用提供了科学依据。更多还原

【Abstract】 To investigate the effects of electrolyzed ozone water on the microbial colonies on grape berry surfaces,this study employed high-throughput sequencing of the 16S rDNA and ITS2 regions to analyze bacterial and fungal colonies on ’Xinya’ grapes at the veraison stage following spray treatments with different concentrations of electrolyzed ozone water,difenoconazole,and water.The results showed that in the electrolyzed ozone water-treated groups,the predominant bacterial phyla on the berry surfaces were Proteobacteria,Actinobacteria,Firmicutes and Bacteroidetes,while the dominant fungal phyla were Ascomycota and Basidiomycota.Key fungal genera included Aspergillus,Cladosporium,Talaromyces,Exobasidium and Penicillium.Although no significant changes in microbial diversity indices were observed,treatment with 5 mg·L^-1 electrolyzed ozone water effectively reduced the relative abundance of harmful pathogenic genera such as Pseudomonas and Penicillium on the grape surfaces,demonstrating superior efficacy compared to difenoconazole treatment.These findings indicate that spraying electrolyzed ozone water can modulate the structure of microbial colonies on grape berries and suppress the proliferation of certain pathogens,providing a scientific basis for its practical application in production.更多还原