【摘要】 WRKY转录因子作为植物特有且保守的一类转录因子，在植物生长发育和次生代谢过程中发挥着重要的调控作用。为发掘参与红花(Carthamus tinctorius)类黄酮代谢调控的WRKY基因，本研究开展了红花基因组水平WRKY家族基因的鉴定分析。基于红花基因组及转录组数据，本研究鉴定出了87个CtWRKY基因，分布在12条染色体上。亚细胞定位预测显示其中绝大多数(85个) CtWRKY基因定位在细胞核中。进化分析显示，87个CtWRKY基因可分为3大族：GroupⅠ/Ⅱ/Ⅲ，其中GroupⅡ又分为5个亚族(GroupⅡa/b/c/d/e)，且占比最高(52.87%)。蛋白基序分析显示CtWRKY蛋白序列中均具有高度保守WRKY结构域(WRKYGQK)。共线性分析表明，红花CtWRKY基因家族成员存在39对片段重复基因。RNA-seq分析结果显示，红花CtWRKY基因家族成员在不同组织存在着明显的表达差异；进一步利用qRT-PCR对红花盛花期花瓣中高表达的9个CtWRKY基因进行检测，发现均可不同程度响应脱落酸(abscisic acid, ABA)和茉莉酸甲酯(methyl jasmonate, MeJA)的诱导。本研究为进一步阐明红花类黄酮生物合成中的WRKY转录因子功能研究奠定基础。更多还原

【Abstract】 WRKY transcription factors are a class of transcription factors that are unique and conserved in plants, playing important regulatory roles in plant growth, development, and secondary metabolism. To explore the WRKY genes involved in the regulation of flavonoid metabolism in safflower(Carthamus tinctorius), the WRKY family genes of safflower, were identified and analyzed at the genome level in this study. Utilizing the genome and transcriptome data of safflower, this study identified 87 CtWRKY genes,distributing across 12 chromosomes. Subcellular localization prediction showed that the vast majority(85)of CtWRKY family members were located in the nucleus. Evolutionary analysis showed that CtWRKY genes could be categorized into 3 groups(GroupⅠ/Ⅱ/Ⅲ) and 5 subgroups(GroupⅡa/b/c/d/e) in GroupⅡ,with the highest number of WRKYs(52.87%). The analysis of protein motif showed that all CtWRKY proteins contained a highly conserved motif WRKYGQK. Collinearity analysis indicated that there were 39pairs of segmental duplication genes in safflower CtWRKY gene family. RNA-seq analysis showed that there were obvious differences in the expression of CtWRKY gene family members in different tissues.Furthermore, the transcriptional profiles of 9 CtWRKY genes highly expressed in full bloom petals were investigated using qRT-PCR analysis. Results showed that a total of 9 CtWRKY genes were differentially expressed in response to abscisic acid(ABA) and methyl jasmonate(MeJA) treatment. This study provides a basis for further elucidating the function of WRKY transcription factors in the biosynthesis of flavonoids in safflower.更多还原