【摘要】 以河南省平顶山市现代农业研发基地为研究对象，建立土壤中苯达松、2甲4氯及氟磺胺草醚和11种磺酰脲类除草剂的快速检测方法，采用液相色谱-串联质谱（LC-MS/MS），通过分步提取结合C₁₈固相萃取净化，使用ACQUITY UPLC?HSS T3柱，以体积分数为1%甲酸水溶液-乙腈为流动相梯度，结合电喷雾正/负离子切换多反应监测模式进行定量分析，采用LC-MS/MS进行定量。试验结果表明：1)14种除草剂的质量分数为0.05～10μg/kg时，进样量与对应的峰面积间呈良好的线性关系，相关系数r≥0.99，加标回收率为88.79%～119.48%;2)非健康生长地块中健康和非健康韭菜根际土中乙草胺的残留量均高于健康生长地块的健康韭菜根际土，表明乙草胺的残留可能与韭菜非健康生长存在潜在关联。本研究为国产LC-MS/MS仪器的应用提供了技术支撑，也为农田除草剂溯源及农产品安全评估提供了技术参考。更多还原

【Abstract】 The Modern Agricultural Research and Development Base in Pingdingshan City,Henan Province,was selected as the research object.A rapid method for the determination of bendazone,2-methyl-4-chlorophenoxyacetic acid,flusulfenoxuron and a total of 11 sulfonylurea herbicides in soil was established.Liquid Chromatography-tandem Mass Spectrometry （LC-MS/MS） technology was adopted.Samples were cleaned-up by stepwise extraction coupled with C₁₈ solid-phase extraction,and quantified by an ACQUITY UPLC?HSS T3 column using a 1% formic acid aqueous solution-acetonitrile as mobile phase gradient.Combined with electrospray positive/negative ion switching multiple reaction monitoring mode,samples were quantitatively analyzed by using LC-MS/MS.The results showed that:1) A good linear relationship was observed between the injection mass fraction and the corresponding peak areas for the 14 herbicides at mass fractions ranging from 0.05 to 10μg/kg,with the correlation coefficient of r≥0.99.The spiked recoveries ranged from 88.79% to 119.48%;2) The residues of acetamiprid in the inter-root soils of healthy and unhealthy leeks in non-healthy growing soil were higher than that in healthy leek inter-root soil,suggesting that ethofumesate residues might be potentially associated with unhealthy leek growth.In conclusion,this study provides technical support for the application of domestic LC-MS/MS instruments,and technical reference for the traceability of herbicides in farmland and the safety assessment of agricultural products.更多还原