【摘要】 目的 通过应用吲哚菁绿(ICG)荧光反染联合术中超声引导技术确定腹腔镜解剖性肝切除术(LAH)肝脏断面，探讨该技术在LAH中应用价值。方法 收集行LAH的122例患者，根据是否应用ICG荧光反染联合术中超声引导技术分为A组(n=58)和B组(n=64),A组为通过传统缺血线确定肝脏断面，B组为应用ICG荧光反染联合术中超声引导技术确定肝脏断面。比较2组患者一般资料、肝门阻断时间、术中出血量、术后24 h腹腔引流量。结果 A组患者肝门阻断时间为95.00(65.00,110.00)min, B组患者为75.00(60.00,101.25)min, 2组患者比较差异有统计学意义(P<0.05)。A组患者术中出血量为270.00(160.00,367.50)mL,B组为200.00(140.00,295.00)mL,2组患者比较差异有统计学意义(P<0.05)。A组患者术后24 h腹腔引流量为180.00(140.00,260.00)mL,B组患者为155.00(82.50,257.50)mL,2组患者比较差异有统计学意义(P<0.05)。2组患者输血比例、肝脏切除体积、术后并发症发生率、住院天数比较，差异均无统计学意义(P>0.05)。结论 LAH中应用ICG荧光反染联合超声引导技术能更准确地确定肝脏断面，缩短了肝门阻断时间，减少了术后出血及术后24 h腹腔引流液，值得临床推广。更多还原

【Abstract】 Objective To determine the liver section of laparoscopic anatomic hepatectomy(LAH) by using indocyanine green(ICG) fluorescence reverse-staining combined with intraoperative ultrasound guidance, and to explore the application value of this technique in LAH.Methods A total of 122 patients who underwent LAH were collected and divided into group A(n=58) and Group B(n=64).In group A,the liver section was determined by traditional ischemic line, while in group B,the liver section was determined by ICG fluorescence reverse-staining combined with intraoperative ultrasound guidance.The general date, the hilar occlusiori time, intraoperative blood loss and abdominal drainage volume 24 hours after operation of the two groups were compared.Results The hilar occlusion time in group A was 95.00(65.00,110.00)min, while it was 75.00(60.00,101.25)min in group B,the difference between the two groups was statistically significant(P<0.05).The intraoperative blood loss in group A was 270.00(160.00,367.50) mL,while it was 200.00(140.00,295.00)mL in group B,the difference between the two groups was statistically significant(P<0.05).The intraperitoneal drainage volume in group B at 24 hours after operation was 155.00(82.50,257.50),which was lower than that in group A at 180.00(140.00,260.00),and the difference was statistically significant(P<0.05).There were no significant differences in the proportion of blood transfusion, the volume of liver resection, the incidence of postoperative complications and the length of hospital stay between the two groups(P>0.05).Conclusion The application of ICG fluorescent reverse-staining combined with ultrasound guidance technology in LAH can more accurately determine the liver section, shorten the time of portal occlusion, reduce postoperative bleeding and 24-hour abdominal drainage after surgery, and is worthy of clinical promotion.更多还原