【摘要】 【目的】明确宁夏枸杞木虱不同田间种群对吡虫啉、氟啶虫胺腈的敏感性及其与体内解毒酶的相关性。【方法】采用点滴法和浸虫法分别测定了宁夏中宁、兴仁和园林场3个枸杞田间种群枸杞木虱成虫和若虫对2种杀虫剂的敏感性，测定了不同田间种群枸杞木虱谷胱甘肽-S-转移酶（GST）、酯酶（EST）和多功能氧化酶（MFO）的活性。【结果】宁夏3个枸杞田间种群枸杞木虱对吡虫啉和氟啶虫胺腈敏感性均不同且中宁种群不同虫态对吡虫啉和氟啶虫胺腈的敏感性均最低；其中：中宁枸杞木虱成虫对吡虫啉和氟啶虫胺腈的LC₅₀值分别为3 751.489 9、970.177 5 mg·L^-1，中宁枸杞木虱若虫对吡虫啉和氟啶虫胺腈的LC₅₀值分别为1 536.658 1、676.350 3 mg·L^-1。中宁种群不同虫态的枸杞木虱3种解毒酶活性均显著高于其他2个种群。相关分析表明，枸杞木虱不同虫态对吡虫啉和氟啶虫胺腈的敏感性水平与其体内的GST、EST和MFO的酶活性之间存在相关性，其中枸杞木虱若虫体内MFO酶活性对吡虫啉和氟啶虫胺腈敏感性水平存在极显著正相关，相关系数为1。【结论】宁夏3个枸杞田间种群枸杞木虱对吡虫啉和氟啶虫胺腈敏感性均不同，中宁种群最低，兴仁种群次之，园林场种群最高，且枸杞木虱对药剂的敏感性和解毒酶活性之间存在明显的正相关。建议因地制宜、合理科学选用杀虫剂，提高枸杞木虱的防治效果。更多还原

【Abstract】 [Objective] To clarify the sensitivity of different field populations of B.gobica to imidacloprid and sulfoxaflor and their correlation with detoxification enzymes in vivo. [Method] The sensitivity of adult and nymphs of B.gobica in three field populations of Zhongning, Xingren and Yuanlinchang in Ningxia to two kinds of insecticides was determined respectively by the spot-drop and dip methods. The activities of glutathione S-transferase（GST）, esterase（EST） and multifunctional oxidase（MFO） in different field populations of B.gobica were determined. [Result] The sensitivities of the three field populations of B.gobica in Ningxia to imidacloprid and sulfoxaflor were all different, and the sensitivities of different insect states of the Zhongning population to imidacloprid and sulfoxaflor were the lowest. Among them, the LC₅₀ values of adult B.gobica in Zhongning to imidacloprid and sulfoxaflor were 3 751.4889 and 970.177 5 mg·L^-1, respectively. The LC₅₀ values of the nymphs of B.gobica in Zhongning to imidacloprid and sulfoxaflor were 1 536.658 1 and 676.350 3 mg·L^-1, respectively. The activities of three detoxification enzymes of different insect states of the Zhongning population of B.gobica were significantly higher than those of the other two populations. Correlation analysis showed that there was a positive correlation between the level of sensitivity of different states of B. gobica to imidacloprid and sulfoxaflor and the enzymatic activities of GST, EST and MFO in their bodies, among them, there was a significant positive correlation between the activity of MFO enzyme and the sensitivity levels of nymphs of B.gobica to imidacloprid and sulfoxaflor, and the correlation coefficient was 1. [Conclusion] The sensitivity of the three field populations of B.gobica in Ningxia to imidacloprid and sulfoxaflor was different. The Zhongning population had the lowest sensitivity, followed by the Xingren population, and the Yuanlinchang population had the highest sensitivity. Moreover, there was a significant positive correlation between the sensitivity of B.gobica to the agents and the activity of detoxification enzymes. It is recommended to select insecticides in a reasonable and scientific way based on local conditions to enhance the control effect of Bactericera gobica.更多还原