【摘要】 【目的】筛选山西省不同大豆品种（系）抗旱性指标，完善抗旱大豆种质资源。【方法】通过盆栽试验对花荚期15份大豆品种（系）进行干旱胁迫处理，测定并计算农艺性状[株高（PH）、茎粗（SD）]、光合参数[气孔导度（G_s）、净光合速率（P_n）、叶绿素相对含量（SPAD）]、生理生化指标[脯氨酸（Pro）含量、可溶性蛋白（Sp）含量、丙二醛（MDA）含量]的抗旱系数（X）、抗旱隶属函数值[B(X)]和抗旱性度量值（D值），运用主成分分析、回归分析对其抗旱性进行综合评价，最后通过大田验证试验计算抗旱指数（DHI）。【结果】干旱胁迫对大豆农艺性状、光合参数、生理生化指标均有显著影响，不同大豆品种（系）各单项指标抗旱系数绝对平均值由高到低为Pro含量>MDA含量>Sp含量>SPAD>SD>PH>P_n>G_s。主成分分析表明，3个主成分贡献率为84.321%，可代表绝大部分数据信息；不同大豆品种（系）的D值依次为Y13>Y4>Y10>Y6>Y9>Y15>Y3>Y11>Y14>Y12>Y1>Y2=Y7>Y8>Y5；回归方程为：D值=-3.459+0.756PH+1.299SD+0.858SPAD+0.382Pro含量+0.693Sp含量+0.397P_n。DHI结果表明，Y13的抗旱性最强，Y5的抗旱性最弱。【结论】长1807(Y13)抗旱性最好，可作为山西省耐旱品种的备选材料；株高、茎粗、叶绿素相对含量、脯氨酸含量、可溶性蛋白含量和净光合速率对抗旱性度量值（D值）具有显著的统计学意义，可作为花荚期不同大豆品种（系）抗旱性指标。更多还原

【Abstract】 【Objective 】To screen drought resistance indicators for different soybean varieties(lines) in Shanxi Province and improve the drought-resistant soybean germplasm resources. 【Methods 】Through pot experiments,15 soybean varieties(lines)at the flowering and podding stage were subjected to drought stress. Drought resistance coefficients(X),drought resistance membership function values [B(X)],and drought resistance measurement values(D-values) were determined and calculated for various agronomic traits[plant height(PH),stem diameter(SD)],photosynthetic parameters[stomatal conductance(G_s),net photosynthetic rate(P_n),relative chlorophyll content(SPAD)],and physiological and biochemical indicators [proline(Pro)content,soluble protein(Sp) content,malondialdehyde(MDA) content]. Principal component analysis and regression analysis were employed to comprehensively evaluate their drought resistance. Finally,a field validation experiment was conducted to calculate the drought resistance index(DHI). 【Results 】Drought stress significantly affected soybean agronomic traits,photosynthetic parameters,and physiological and biochemical indicators. The absolute mean values of drought resistance coefficients for individual indicators across different soybean varieties(lines),from highest to lowest,were:Pro content>MDA content>Sp content>SPAD>SD>PH >P_n>G_s. Principal component analysis revealed that the first three principal components contributed 84.321% of the total variance,representing the majority of the data information. The D-values for different soybean varieties(lines) were,in descending order:Y13>Y4>Y10>Y6>Y9>Y15>Y3>Y11>Y14>Y12>Y1>Y2 =Y7>Y8>Y5.The regression equation was:D-value=-3.459+0.756PH+1.299SD+0.858SPAD+0.382Pro content+0.693Sp content+0.397P_n.DHI results indicated that Y13 exhibited the strongest drought resistance,while Y5 showed the weakest. 【Conclusion】Chang1807(Y13) demonstrates the best drought resistance and can serve as a potential candidate for drought-tolerant varieties in Shanxi Province. Plant height,stem diameter,relative chlorophyll content,proline content,soluble protein content,and net photosynthetic rate exhibit significant statistical correlations with the drought resistance measurement value(D-value),thus serving as effective drought resistance indicators for different soybean varieties(lines) at the flowering and podding stage.更多还原