【摘要】 研究基于高效液相色谱技术，建立了一种猪用配合饲料中6种药物成分的分析方法，实现复杂基质下痕量药物的精准检测。结果表明，当采用提取溶剂为乙腈、甲酸和水，体积比为75∶0.1∶25时，各药物回收率为92.3%～96.8%,方法检出限为0.5～3.0μg/kg,相对标准偏差RSD均低于5%,满足产业检测需求。同时，在教学实践中，以饲料样品为载体，引导学生完成溶剂选择梯度洗脱优化加标验证全流程操作。学生可以操作Agilent 1 260等产业标准设备，并通过不同溶剂体系的回收率差异掌握结构性质匹配原则。更多还原

【Abstract】 Based on high performance liquid chromatography technology, this study established an analytical method for 6 pharmaceutical components in compound feeds for pigs, enabling accurate detection of trace drugs in complex matrices.The results showed that when the extraction solvent was acetonitrile, formic acid, and water with a volume ratio of 75∶0.1∶25,the recovery rate of each drug ranged from 92.3% to 96.8%,the method detection limit was 0.5–3.0 μg/kg, and the relative standard deviation(RSD) was all below 5%,which meets the needs of industrial testing.Meanwhile, in teaching practice, with feed samples as the carrier, students were guided to complete the whole process of solvent selection, gradient elution optimization, and spiked verification.Students can operate industry-standard equipment such as Agilent 1 260,and master the principle of matching structural properties through the recovery rate differences of different solvent systems.更多还原