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响应面法优化桑叶蛋白提取工艺研究

Research on the Extraction Process of Mulberry Leaf Protein Optimized by Response Surface Methodology

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【作者】 崔琛; 高菁旋; 番成砖; 李林; 李平平; 杨文; 李金见;

【Author】 CUI Chen;GAO Jingxuan;FAN Chengzhuan;LI Lin;LI Pingping;YANG Wen;LI Jinjian;Institute of Sericulture and Apiculture,Yunnan Academy of Agricultural Sciences;Baoshan Academy of Agricultural Sciences;

【通讯作者】 李金见;

【机构】 云南省农业科学院蚕桑蜜蜂研究所; 保山市农业科学研究院;

【摘要】 以云南蒙自饲料桑叶为原料,利用微波辅助水溶液提取桑叶蛋白质,采用单因素试验结合响应面法优化微波辅助提取桑叶蛋白质。结果表明,最佳工艺提取条件为微波功率472 W,料液比1∶48(g∶mL),微波时间38 s,桑叶蛋白质提取率达到28.47%。该方法可减少蛋白质提取时间,避免酸、碱化学试剂添加造成污染,同时提高桑叶蛋白质提取效率,为我国桑叶资源开发利用提供一定试验依据。

【Abstract】 Using Yunnan Mengzi fodder mulberry leaves as raw materials,microwave-assisted aqueous solution extraction was employed to obtain mulberry leaf protein, and single-factor tests combined with response surface methodology were used to optimize the microwave-assisted extraction process. The results showed that the optimal extraction conditions were as follows:microwave power was 472 W,the solid-liquid ratio was 1∶48(g∶mL),the microwave time was 38 s,and the protein extraction rate reached 28.47%. This method reduced the protein extraction time,avoided the pollution caused by the addition of acid and alkali chemical reagents,improved the extraction efficiency of mulberry leaf protein,and provided certain data support and theoretical guarantee for the development and utilization of mulberry leaf resources in China.

【基金】 向仲怀院士工作站(202305AF150166)
  • 【文献出处】 农产品加工 ,Agricultural Products Processing , 编辑部邮箱 ,2025年24期
  • 【分类号】TQ936
  • 【下载频次】142
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