【摘要】 为研究植物乳杆菌（LP）、枯草芽孢杆菌（BS）及其复合处理对紫花苜蓿青贮饲料的营养品质和发酵特性的影响，试验设置有4个处理组，分别为对照组（CK）、植物乳杆菌组LP(1×10~6CFU/g FW)、枯草芽孢杆菌组BS(1×10~6CFU/g FW)和植物乳杆菌+枯草芽孢杆菌组LPBS(1×10~6CFU/g FW+1×10~6CFU/g FW)。经过不同处理的样本分别在真空密封聚乙烯袋中保存1,7,15,30,60 d。结果表明，与对照组和其他处理组相比，LP组处理的青贮饲料具有较高的乳酸含量和较少的蛋白质分解，而BS组处理的青贮饲料具有较低的中性洗涤纤维、酸性洗涤纤维和半纤维素含量，以及较高的可溶性碳水化合物含量，提高了木质纤维的降解率，且在增加乳酸浓度和降低结构性碳水化合物含量方面，植物乳杆菌和枯草芽孢杆菌可能具有一定的协同作用。与对照组相比，不同处理均显著提高了乳酸菌的数量（p<0.05），这也是在青贮期间霉菌和酵母菌的数量迅速降低的主要原因。添加植物乳杆菌和枯草芽孢杆菌有助于生产优质的苜蓿青贮饲料，为微生物制剂的应用提供了一种简单可行的方法。更多还原

【Abstract】 The purpose of this experiment was to study the effects of Lactobacillus plantarum(LP),Bacillus subtilis(BS)and their combinations on the nutritional quality and fermentation characteristics of alfalfa silage. There were four treatments:control(CK),Lactobacillus plantarum LP(1×10~6 CFU/g FW),Bacillus subtilis BS(1×10~6 CFU/g FW) and Lactobacillus plantarum + Bacillus subtilis LPBS(1×10~6 CFU/g FW + 1×10~6 CFU/g FW). The samples with different treatments were stored in vacuum-sealed polyethylene bags for 1,7,15,30,60 days,respectively. The results showed that compared with the control and other treatments,the silage treated with LP had higher content of lactic acid and less decomposition of crude protein,while the silage treated with BS had lower contents of neutral detergent fiber,acid detergent fiber and hemicellulose and higher content of water-soluble carbohydrate,which increased the degradation rate of lignofiber. Lactobacillus plantarum and Bacillus subtilis had synergistic effect in increasing the concentration of lactic acid and reducing the content of structural carbohydrates. Compared with the control, different treatments significantly increased the number of lactic acid bacteria,which was the main reason for the rapid decrease of mold and yeast during silage. The experimental results showed that the addition of LP and BS was helpful to produce high quality alfalfa feed and provided a simple and feasible method for the application of microbial preparation.更多还原