【摘要】 以黑枸杞为原料，花青素和多酚含量为检测指标，通过单因素试验和响应面法探究了复合酶解最佳条件。结果表明，最佳工艺条件为纤维素酶和果胶酶配比6.89∶3.11，复合酶添加量15.02%，酶解时间2.45 h，酶解温度30℃，在此条件下花青素提取量为（7.02±0.23） mg/g，多酚提取量为（34.68±0.22） mg/g。同时，测定添加复合酶和未添加复合酶的黑枸杞果浆的抗氧化活性变化，结果显示酶解后黑枸杞果浆的抗氧化活性得到提高。更多还原

【Abstract】 In this experiment,Lycium ruthenicum Murr. was used as the raw material,and the contents of anthocyanins and polyphenols were used as detection indicators. The optimal conditions for composite enzyme hydrolysis were explored through single-factor experiments and response surface methodology. The results showed that the optimal process conditions were:the ratio of cellulase to pectinase was 6.89∶3.11,the addition amount of composite enzyme was 15.02%,the hydrolysis time was2.45 h,and the temperature was 30 ℃. Under these conditions,the results obtained were:anthocyanin content of(7.02±0.23) mg/g and polyphenol content of(34.68±0.22) mg/g. Simultaneously,the changes in antioxidant activity of Lycium ruthenicum Murr. and blueberry juice with and without the addition of composite enzyme were determined. The results showed that the antioxidant activity of Lycium ruthenicum Murr. juice was improved after enzyme hydrolysis.更多还原